PIPs

Phosphatidylinositol (P-0###)

1. Barylko, B., Gerber, S. H., Binns, D. D., Grichine, N., Khvotchev, M., Sudhof, T. C., and Albanesi, J. P., A Novel Family of Phosphatidylinositol 4-Kinases Conserved from Yeast to Humans, J Biol Chem, 276, 7705 (2001).
2. Guo, J., Wenk, M. R., Pellegrini, L., Onofri, F., Benfenati, F., and De Camilli, P., Phosphatidylinositol 4-kinase type IIalpha is responsible for the phosphatidylinositol 4-kinase activity associated with synaptic vesicles, Proc Natl Acad Sci U S A, 100, 3995 (2003).
3. Hama, H., Torabinejad, J., Prestwich, G. D., and DeWald, D. B., Measurement and Immunofluorescence of Cellular Phosphoinositides, in Methods in Molecular Biology: Signal Transduction Protocols, Vol. 284, Dickson, R. C., Ed., Humana Press Inc., Totowa, NJ, pp. 243 (2003).
4. He, F., Mao, M., and Wensel, T. G., Enhancement of phototransduction g protein-effector interactions by phosphoinositides, J Biol Chem, 279, 8986 (2004).
5. Heras, B., and Drobak, B. K., PARF-1: an Arabidopsis thaliana FYVE-domain protein displaying a novel eukaryotic domain structure and phosphoinositide affinity, J Exp Bot, 53, 565 (2002).
6. Hodgkin, M. N., Masson, M. R., Powner, D., Saqib, K. M., Ponting, C. P., and Wakelam, M. J., Phospholipase D regulation and localisation is dependent upon a phosphatidylinositol 4,5-biphosphate-specific PH domain, Curr Biol, 10, 43 (2000).
7. Huang, Z., Guo, X. X., Chen, S. X., Alvarez, K. M., Bell, M. W., and Anderson, R. E., Regulation of Type II Phosphatidylinositol Phosphate Kinase by Tyrosine Phosphorylation in Bovine Rod Outer Segments, Biochemistry, 40, 4550 (2001).
8. Johnson, L. M., James, K. M., Chamberlain, M. D., and Anderson, D. H., Identification of Key Residues in the A-Raf Kinase Important for Phosphoinositide Lipid Binding Specificity, Biochemistry, 44, 3432 (2005).
9. Kalthoff, C., Groos, S., Kohl, R., Mahrhold, S., and Ungewickell, E. J., Clint: a novel clathrin-binding ENTH-domain protein at the Golgi, Mol Biol Cell, 13, 4060 (2002).
10. Kobayashi, M., Mutharasan, R. K., Feng, J., Roberts, M. F., and Lomasney, J. W., Identification of hydrophobic interactions between proteins and lipids: free fatty acids activate phospholipase C delta1 via allosterism, Biochemistry, 43, 7522 (2004).
11. Kutateladze, T., and Overduin, M., Structural Mechanism of Endosome Docking by the FYVE Domain, Science, 291, 1793 (2001).
12. Marcus, S. L., Wenk, M. R., Steele-Mortimer, O., and Finlay, B. B., A synaptojanin-homologous region of Salmonella typhimurium SigD is essential for inositol phosphatase activity and Akt activation, FEBS Lett, 494, 201 (2001).
13. Merlot, S., Meili, R., Pagliarini, D. J., Maehama, T., Dixon, J. E., and Firtel, R. A., A PTEN-related 5-phosphatidylinositol phosphatase localized in the Golgi, J Biol Chem, 278, 39866 (2003).
14. Osborne, S. L., Thomas, C. L., Gschmeissner, S., and Schiavo, G., Nuclear PtdIns(4,5)P2 assembles in a mitotically regulated particle involved in pre-mRNA splicing, J Cell Sci, 114, 2501 (2001).
15. Redfern, D. A., and Gericke, A., Domain formation in phosphatidylinositol monophosphate/phosphatidylcholine mixed vesicles, Biophys J, 86, 2980 (2004).
16. Russo, C., Gao, Y., Mancini, P., Vanni, C., Porotto, M., Falasca, M., Torrisi, M. R., Zheng, Y., and Eva, A., Modulation of oncogenic DBL activity by phosphoinositol phosphate binding to pleckstrin homology domain, J Biol Chem, 276, 19524 (2001).
17. Sbrissa, D., Ikonomov, O. C., and Shisheva, A., PIKfyve, a mammalian ortholog of yeast Fab1p lipid kinase, synthesizes 5-phosphoinositides. Effect of insulin, J Biol Chem, 274, 21589 (1999).

All 3'PIPs (P-3###)

1. Abram, C. L., Seals, D. F., Pass, I., Salinsky, D., Maurer, L., Roth, T. M., and Courtneidge, S. A., The Adaptor Protein Fish Associates with Members of the ADAMs Family and Localizes to Podosomes of Src-transformed Cells, J Biol Chem, 278, 16844 (2003).
2. Ahn, J.-Y., Rong, R., Liu, X., and Ye, K., PIKE/nuclear PI 3-kinase signaling mediates the antiapoptotic actions of NGF in the nucleus, EMBO J., sj.emboj.7600392 (2004).
3. Banfic, H., Downes, C. P., and Rittenhouse, S. E., Biphasic activation of PKBalpha/Akt in platelets. Evidence for stimulation both by phosphatidylinositol 3,4-bisphosphate, produced via a novel pathway, and by phosphatidylinositol 3,4,5-trisphosphate, J Biol Chem, 273, 11630 (1998).
4. Barylko, B., Gerber, S. H., Binns, D. D., Grichine, N., Khvotchev, M., Sudhof, T. C., and Albanesi, J. P., A Novel Family of Phosphatidylinositol 4-Kinases Conserved from Yeast to Humans, J Biol Chem, 276, 7705 (2001).
5. Begley, M. J., Taylor, G. S., Kim, S. A., Veine, D. M., Dixon, J. E., and Stuckey, J. A., Crystal structure of a phosphoinositide phosphatase, MTMR2: insights into myotubular myopathy and Charcot-Marie-Tooth syndrome, Mol Cell, 12, 1391 (2003).
6. Blader, I. J., Cope, M. J., Jackson, T. R., Profit, A. A., Greenwood, A. F., Drubin, D. G., Prestwich, G. D., and Theibert, A. B., GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro, Mol Biol Cell, 10, 581 (1999).
7. Bravo, J., Karathanassis, D., Pacold, C. M., Pacold, M. E., Ellson, C. D., Anderson, K. E., Butler, P. J., Lavenir, I., Perisic, O., Hawkins, P. T., Stephens, L., and Williams, R. L., The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate, Mol Cell, 8, 829 (2001).
8. Caffrey, J. J., Darden, T., Wenk, M. R., and Shears, S. B., Expanding coincident signaling by PTEN through its inositol 1,3,4,5,6- pentakisphosphate 3-phosphatase activity, FEBS Lett, 499, 6 (2001).
9. Campbell, R. B., Liu, F., and Ross, A. H., Allosteric activation of PTEN phosphatase by phosphatidylinositol 4,5-bisphosphate, J Biol Chem, 278, 33617 (2003).
10. Chang, J. D., Field, S. J., Rameh, L. E., Carpenter, C. L., and Cantley, L. C., Identification and characterization of a phosphoinositide phosphate kinase homolog, J Biol Chem, 279, 11672 (2004).
11. Cheever, M. L., Sato, T. K., de Beer, T., Kutateladze, T. G., Emr, S. D., and Overduin, M., Phox domain interaction with PtdIns(3)P targets the Vam7 t-SNARE to vacuole membranes, Nat Cell Biol, 3, 613 (2001).
12. Chellaiah, M. A., Biswas, R. S., Yuen, D., Alvarez, U. M., and Hruska, K. A., Phosphatidylinositol 3,4,5-trisphosphate directs association of Src homology 2-containing signaling proteins with gelsolin, J Biol Chem, 276, 47434 (2001).
13. Chen, R., Kang, V. H., Chen, J., Shope, J. C., Torabinejad, J., DeWald, D. B., and Prestwich, G. D., A monoclonal antibody to visualize PtdIns(3,4,5)P(3) in cells, J Histochem Cytochem, 50, 697 (2002).
14. Chi, Y., Zhou, B., Wang, W.-Q., Chung, S.-K., Kwon, Y.-U., Ahn, Y.-H., Chang, Y.-T., Tsujishita, Y., Hurley, J. H., and Zhang, Z.-Y., Comparative mechanistic and substrate specificity study of inositol polyphosphate 5-phosphatases SPsynaptojanin and SHIP2, J. Biol. Chem., M406416200 (2004).
15. Clarke, J. H., Lipid signalling: picking out the PIPs, Curr Biol, 13, R815 (2003).
16. Corbalan-Garcia, S., Garcia-Garcia, J., Rodriguez-Alfaro, J. A., and Gomez-Fernandez, J. C., A new phosphatidylinositol 4,5-bisphosphate-binding site located in the C2 domain of protein kinase Calpha, J Biol Chem, 278, 4972 (2003).
17. Corgan, A. M., Singleton, C., Santoso, C. B., and Greenwood, J. A., Phosphoinositides differentially regulate alpha-actinin flexibility and function, Biochem J, 378, 1067 (2004).
18. Crottet, P., Meyer, D. M., Rohrer, J., and Spiess, M., ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5- bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes, Mol Biol Cell, 13, 3672 (2002).
19. Das, S., Dixon, J. E., and Cho, W., Membrane-binding and activation mechanism of PTEN, Proc Natl Acad Sci U S A, 100, 7491 (2003).
20. Deak, M., Casamayor, A., Currie, R. A., Downes, C. P., and Alessi, D. R., Characterisation of a plant 3-phosphoinositide-dependent protein kinase- 1 homologue which contains a pleckstrin homology domain, FEBS Lett, 451, 220 (1999).
21. Dowler, S., Currie, R. A., Downes, C. P., and Alessi, D. R., DAPP1: a dual adaptor for phosphotyrosine and 3-phosphoinositides, Biochem J, 342, 7 (1999).
22. Elge, S., Brearley, C., Xia, H. J., Kehr, J., Xue, H. W., and Mueller-Roeber, B., An Arabidopsis inositol phospholipid kinase strongly expressed in procambial cells: synthesis of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 in insect cells by 5-phosphorylation of precursors, Plant J, 26, 561 (2001).
23. Fleming, I. N., Elliott, C. M., Buchanan, F. G., Downes, C. P., and Exton, J. H., Ca2+/calmodulin-dependent protein kinase II regulates Tiam1 by reversible protein phosphorylation, J Biol Chem, 274, 12753 (1999).
24. Fleming, I. N., Gray, A., and Downes, C. P., Regulation of the Rac1-specific exchange factor Tiam1 involves both phosphoinositide 3-kinase-dependent and -independent components, Biochem J, 351, 173 (2000).
25. Fraley, T. S., Tran, T. C., Corgan, A. M., Nash, C. A., Hao, J., Critchley, D. R., and Greenwood, J. A., Phosphoinositide binding inhibits alpha -actinin bundling activity, J Biol Chem, 24, 24 (2003).
26. Friant, S., Pecheur, E. I., Eugster, A., Michel, F., Lefkir, Y., Nourrisson, D., and Letourneur, F., Ent3p Is a PtdIns(3,5)P2 effector required for protein sorting to the multivesicular body, Dev Cell, 5, 499 (2003).
27. Fuster, D., Moe, O. W., and Hilgemann, D. W., Lipid- and mechanosensitivities of sodium/hydrogen exchangers analyzed by electrical methods, Proc Natl Acad Sci U S A, 101, 10482 (2004).
28. Gaidarov, I., Krupnick, J. G., Falck, J. R., Benovic, J. L., and Keen, J. H., Arrestin function in G protein-coupled receptor endocytosis requires phosphoinositide binding, Embo J, 18, 871 (1999).
29. Georgescu, M. M., Kirsch, K. H., Akagi, T., Shishido, T., and Hanafusa, H., The tumor-suppressor activity of PTEN is regulated by its carboxyl- terminal region, Proc Natl Acad Sci U S A, 96, 10182 (1999).
30. Gillooly, D. J., Morrow, I. C., Lindsay, M., Gould, R., Bryant, N. J., Gaullier, J. M., Parton, R. G., and Stenmark, H., Localization of phosphatidylinositol 3-phosphate in yeast and mammalian cells [In Process Citation], Embo J, 19, 4577 (2000).
31. Gozani, O., Karuman, P., Jones, D. R., Ivanov, D., Cha, J., Lugovskoy, A. A., Baird, C. L., Zhu, H., Field, S. J., Lessnick, S. L., Villasenor, J., Mehrotra, B., Chen, J., Rao, V. R., Brugge, J. S., Ferguson, C. G., Payrastre, B., Myszka, D. G., Cantley, L. C., Wagner, G., Divecha, N., Prestwich, G. D., and Yuan, J., The PHD finger of the chromatin-associated protein ING2 functions as a nuclear phosphoinositide receptor, Cell, 114, 99 (2003).
32. Guo, J., Wenk, M. R., Pellegrini, L., Onofri, F., Benfenati, F., and De Camilli, P., Phosphatidylinositol 4-kinase type IIalpha is responsible for the phosphatidylinositol 4-kinase activity associated with synaptic vesicles, Proc Natl Acad Sci U S A, 100, 3995 (2003).
33. Hama, H., Torabinejad, J., Prestwich, G. D., and DeWald, D. B., Measurement and Immunofluorescence of Cellular Phosphoinositides, in Methods in Molecular Biology: Signal Transduction Protocols, Vol. 284, Dickson, R. C., Ed., Humana Press Inc., Totowa, NJ, pp. 243 (2003).
34. Han, S. Y., Kato, H., Kato, S., Suzuki, T., Shibata, H., Ishii, S., Shiiba, K., Matsuno, S., Kanamaru, R., and Ishioka, C., Functional evaluation of PTEN missense mutations using in vitro phosphoinositide phosphatase assay, Cancer Res, 60, 3147 (2000).
35. He, F., Mao, M., and Wensel, T. G., Enhancement of phototransduction g protein-effector interactions by phosphoinositides, J Biol Chem, 279, 8986 (2004).
36. Heras, B., and Drobak, B. K., PARF-1: an Arabidopsis thaliana FYVE-domain protein displaying a novel eukaryotic domain structure and phosphoinositide affinity, J Exp Bot, 53, 565 (2002).
37. Hodgkin, M. N., Masson, M. R., Powner, D., Saqib, K. M., Ponting, C. P., and Wakelam, M. J., Phospholipase D regulation and localisation is dependent upon a phosphatidylinositol 4,5-biphosphate-specific PH domain, Curr Biol, 10, 43 (2000).
38. Huang, Z., Guo, X. X., Chen, S. X., Alvarez, K. M., Bell, M. W., and Anderson, R. E., Regulation of Type II Phosphatidylinositol Phosphate Kinase by Tyrosine Phosphorylation in Bovine Rod Outer Segments, Biochemistry, 40, 4550 (2001).
39. Iijima, M., Huang, Y. E., Luo, H. R., Vazquez, F., and Devreotes, P. N., Novel Mechanism of PTEN Regulation by Its Phosphatidylinositol 4,5-Bisphosphate Binding Motif Is Critical for Chemotaxis, J Biol Chem, 279, 16606 (2004).
40. Ikonomov, O. C., Sbrissa, D., Mlak, K., Kanzaki, M., Pessin, J., and Shisheva, A., Functional dissection of lipid and protein kinase signals of PIKfyve reveals the role of PtdIns 3,5-P2 production for endomembrane integrity, J Biol Chem, 277, 9206 (2002).
41. Jenco, J. M., Rawlingson, A., Daniels, B., and Morris, A. J., Regulation of phospholipase D2: selective inhibition of mammalian phospholipase D isoenzymes by alpha- and beta-synucleins, Biochemistry, 37, 4901 (1998).
42. Johnson, L. M., James, K. M., Chamberlain, M. D., and Anderson, D. H., Identification of Key Residues in the A-Raf Kinase Important for Phosphoinositide Lipid Binding Specificity, Biochemistry, 44, 3432 (2005).
43. Jones, S. M., Klinghoffer, R., Prestwich, G. D., Toker, A., and Kazlauskas, A., PDGF induces an early and a late wave of PI 3-kinase activity, and only the late wave is required for progression through G1, Curr Biol, 9, 512 (1999).
44. Jones, S. M., and Kazlauskas, A., Growth-factor-dependent mitogenesis requires two distinct phases of signalling, Nat Cell Biol, 3, 165 (2001).
45. Kalthoff, C., Groos, S., Kohl, R., Mahrhold, S., and Ungewickell, E. J., Clint: a novel clathrin-binding ENTH-domain protein at the Golgi, Mol Biol Cell, 13, 4060 (2002).
46. Karathanassis, D., Stahelin, R. V., Bravo, J., Perisic, O., Pacold, C. M., Cho, W., and Williams, R. L., Binding of the PX domain of p47(phox) to phosphatidylinositol 3,4- bisphosphate and phosphatidic acid is masked by an intramolecular interaction, Embo J, 21, 5057 (2002).
47. Kim, S. A., Taylor, G. S., Torgersen, K. M., and Dixon, J. E., Myotubularin and MTMR2, phosphatidylinositol 3-phosphatases mutated in myotubular myopathy and type 4B Charcot-Marie-Tooth disease, J Biol Chem, 277, 4526 (2002).
48. Kruger, J. M., Fukushima, T., Cherepanov, V., Borregaard, N., Loeve, C., Shek, C., Sharma, K., Tanswell, A. K., Chow, C. W., and Downey, G. P., Protein-tyrosine phosphatase MEG2 is expressed by human neutrophils. Localization to the phagosome and activation by polyphosphoinositides, J Biol Chem, 277, 2620 (2002).
49. Krylova, I. N., Sablin, E. P., Moore, J., Xu, R. X., Waitt, G. M., MacKay, J. A., Juzumiene, D., Bynum, J. M., Madauss, K., Montana, V., Lebedeva, L., Suzawa, M., Williams, J. D., Williams, S. P., Guy, R. K., Thornton, J. W., Fletterick, R. J., Willson, T. M., and Ingraham, H. A., Structural analyses reveal phosphatidyl inositols as ligands for the NR5 orphan receptors SF-1 and LRH-1, Cell, 120, 343 (2005).
50. Kunz, J., Wilson, M. P., Kisseleva, M., Hurley, J. H., Majerus, P. W., and Anderson, R. A., The activation loop of phosphatidylinositol phosphate kinases determines signaling specificity, Mol Cell, 5, 1 (2000).
51. Kunz, J., Fuelling, A., Kolbe, L., and Anderson, R. A., Stereo-specific substrate recognition by phosphatidylinositol phosphate kinases is swapped by changing a single amino acid residue, J Biol Chem, 277, 5611 (2002).
52. Kutateladze, T. G., Ogburn, K. D., Watson, W. T., de Beer, T., Emr, S. D., Burd, C. G., and Overduin, M., Phosphatidylinositol 3-phosphate recognition by the FYVE domain, Mol Cell, 3, 805 (1999).
53. Kutateladze, T. G., Capelluto, D. G., Ferguson, C. G., Cheever, M. L., Kutateladze, A. G., Prestwich, G. D., and Overduin, M., Multivalent mechanism of membrane insertion by the FYVE domain, J Biol Chem, 279, 3050 (2004).
54. Le Blanc, C., Mironneau, C., Barbot, C., Henaff, M., Bondeva, T., Wetzker, R., and Macrez, N., Regulation of Vascular L-type Ca2+ Channels by Phosphatidylinositol 3,4,5-Trisphosphate, Circ. Res., 95, 300 (2004).
55. Lee, J. O., Yang, H., Georgescu, M. M., Di Cristofano, A., Maehama, T., Shi, Y., Dixon, J. E., Pandolfi, P., and Pavletich, N. P., Crystal structure of the PTEN tumor suppressor: implications for its phosphoinositide phosphatase activity and membrane association, Cell, 99, 323 (1999).
56. Lee, E., Marcucci, M., Daniell, L., Pypaert, M., Weisz, O. A., Ochoa, G. C., Farsad, K., Wenk, M. R., and De Camilli, P., Amphiphysin 2 (Bin1) and T-tubule biogenesis in muscle, Science, 297, 1193 (2002).
57. Lee, S. J., Xu, H., Kang, L. W., Amzel, L. M., and Montell, C., Light adaptation through phosphoinositide-regulated translocation of Drosophila visual arrestin, Neuron, 39, 121 (2003).
58. Li, X., Rivas, M. P., Fang, M., Marchena, J., Mehrotra, B., Chaudhary, A., Feng, L., Prestwich, G. D., and Bankaitis, V. A., Analysis of oxysterol binding protein homologue Kes1p function in regulation of Sec14p-dependent protein transport from the yeast Golgi complex, J Cell Biol, 157, 63 (2002).
59. Lu, Y., Yu, Q., Liu, J. H., Zhang, J., Wang, H., Koul, D., McMurray, J. S., Fang, X., Yung, W. K., Siminovitch, K. A., and Mills, G. B., Src family protein-tyrosine kinases alter the function of PTEN to regulate phosphatidylinositol 3-kinase/AKT cascades, J Biol Chem, 278, 40057 (2003).
60. Macia, E., Paris, S., and Chabre, M., Binding of the PH and polybasic C-terminal domains of ARNO to phosphoinositides and to acidic lipids, Biochemistry, 39, 5893 (2000).
61. Maffucci, T., Razzini, G., Ingrosso, A., Chen, H., Iacobelli, S., Sciacchitano, S., Quon, M. J., and Falasca, M., Role of pleckstrin homology domain in regulating membrane targeting and metabolic function of insulin receptor substrate 3, Mol Endocrinol, 17, 1568 (2003).
62. Marcus, S. L., Wenk, M. R., Steele-Mortimer, O., and Finlay, B. B., A synaptojanin-homologous region of Salmonella typhimurium SigD is essential for inositol phosphatase activity and Akt activation, FEBS Lett, 494, 201 (2001).
63. Mari, M., Macia, E., Le Marchand-Brustel, Y., and Cormont, M., Role of the FYVE finger and the RUN domain for the subcellular localization of Rabip4, J Biol Chem, 276, 42501 (2001).
64. Mehrotra, B., Myszka, D. G., and Prestwich, G. D., Binding kinetics and ligand specificity for the interactions of the C2B domain of synaptogmin II with inositol polyphosphates and phosphoinositides, Biochemistry, 39, 9679 (2000).
65. Merino-Trigo, A., Kerr, M. C., Houghton, F., Lindberg, A., Mitchell, C., Teasdale, R. D., and Gleeson, P. A., Sorting nexin 5 is localized to a subdomain of the early endosomes and is recruited to the plasma membrane following EGF stimulation, J Cell Sci, 117, 6413 (2004).
66. Nagata, Y., Lan, K. H., Zhou, X., Tan, M., Esteva, F. J., Sahin, A. A., Klos, K. S., Li, P., Monia, B. P., Nguyen, N. T., Hortobagyi, G. N., Hung, M. C., and Yu, D., PTEN activation contributes to tumor inhibition by trastuzumab, and loss of PTEN predicts trastuzumab resistance in patients, Cancer Cell, 6, 117 (2004).
67. Niebuhr, K., Giuriato, S., Pedron, T., Philpott, D. J., Gaits, F., Sable, J., Sheetz, M. P., Parsot, C., Sansonetti, P. J., and Payrastre, B., Conversion of PtdIns(4,5)P(2) into PtdIns(5)P by the S.flexneri effector IpgD reorganizes host cell morphology, Embo J, 21, 5069 (2002).
68. Oganesian, A., Poot, M., Daum, G., Coats, S. A., Wright, M. B., Seifert, R. A., and Bowen-Pope, D. F., Protein tyrosine phosphatase RQ is a phosphatidylinositol phosphatase that can regulate cell survival and proliferation, Proc Natl Acad Sci U S A, 100, 7563 (2003).
69. Ortiz, P. A., Hong, N. J., and Garvin, J. L., Luminal flow induces eNOS activation and translocation in the rat thick ascending limb. II. Role of PI3-kinase and Hsp90, Am J Physiol Renal Physiol, 287, F281 (2004).
70. Osborne, S. L., Thomas, C. L., Gschmeissner, S., and Schiavo, G., Nuclear PtdIns(4,5)P2 assembles in a mitotically regulated particle involved in pre-mRNA splicing, J Cell Sci, 114, 2501 (2001).
71. Ozaki, S., DeWald, D. B., Shope, J. C., Chen, J., and Prestwich, G. D., Intracellular delivery of phosphoinositides and inositol phosphates using polyamine carriers, Proc Natl Acad Sci U S A, 97, 11286 (2000).
72. Peterman, T. K., Ohol, Y. M., McReynolds, L. J., and Luna, E. J., Patellin1, a Novel Sec14-Like Protein, Localizes to the Cell Plate and Binds Phosphoinositides, Plant Physiology, pp.104.045369 (2004).
73. Rao, V. D., Misra, S., Boronenkov, I. V., Anderson, R. A., and Hurley, J. H., Structure of type IIbeta phosphatidylinositol phosphate kinase: a protein kinase fold flattened for interfacial phosphorylation, Cell, 94, 829 (1998).
74. Rao, V. R., Corradetti, M. N., Chen, J., Peng, J., Yuan, J., Prestwich, G. D., and Brugge, J. S., Expression cloning of protein targets for 3-phosphorylated phosphoinositides, J Biol Chem, 274, 37893 (1999).
75. Razzini, G., Ingrosso, A., Brancaccio, A., Sciacchitano, S., Esposito, D. L., and Falasca, M., Different subcellular localization and phosphoinositides binding of insulin receptor substrate protein pleckstrin homology domains, Mol Endocrinol, 14, 823 (2000).
76. Redfern, D. A., and Gericke, A., Domain formation in phosphatidylinositol monophosphate/phosphatidylcholine mixed vesicles, Biophys J, 86, 2980 (2004).
77. Rohacs, T., Lopes, C., Mirshahi, T., Jin, T., Zhang, H., and Logothetis, D. E., Assaying phosphatidylinositol bisphosphate regulation of potassium channels, Methods Enzymol, 345, 71 (2002).
78. Rohacs, T., Lopes, C. M., Jin, T., Ramdya, P. P., Molnar, Z., and Logothetis, D. E., Specificity of activation by phosphoinositides determines lipid regulation of Kir channels, Proc Natl Acad Sci U S A, 100, 745 (2003).
79. Russo, C., Gao, Y., Mancini, P., Vanni, C., Porotto, M., Falasca, M., Torrisi, M. R., Zheng, Y., and Eva, A., Modulation of oncogenic DBL activity by phosphoinositol phosphate binding to pleckstrin homology domain, J Biol Chem, 276, 19524 (2001).
80. Sanchez, T., Thangada, S., Wu, M. T., Kontos, C. D., Wu, D., Wu, H., and Hla, T., PTEN as an effector in the signaling of antimigratory G protein-coupled receptor, Proc Natl Acad Sci U S A, 102, 4312 (2005).
81. Sankaran, V. G., Klein, D. E., Sachdeva, M. M., and Lemmon, M. A., High-affinity binding of a fyve domain to phosphatidylinositol 3- phosphate requires intact phospholipid but not fyve domain oligomerization, Biochemistry, 40, 8581 (2001).
82. Sbrissa, D., Ikonomov, O. C., and Shisheva, A., PIKfyve, a mammalian ortholog of yeast Fab1p lipid kinase, synthesizes 5-phosphoinositides. Effect of insulin, J Biol Chem, 274, 21589 (1999).
84. Scheid, M. P., Huber, M., Damen, J. E., Hughes, M., Kang, V., Neilsen, P., Prestwich, G. D., Krystal, G., and Duronio, V., Phosphatidylinositol (3,4,5)P3 is essential but not sufficient for protein kinase B (PKB) activation; phosphatidylinositol (3,4)P2 is required for PKB phosphorylation at Ser-473: studies using cells from SH2-containing inositol-5-phosphatase knockout mice, J Biol Chem, 277, 9027 (2002).
85. Schwartzbauer, G., and Robbins, J., The tumor suppressor gene PTEN can regulate cardiac hypertrophy and survival, J Biol Chem, 276, 35786 (2001).
86. Selengut, J. D., and Levine, R. L., MDP-1: A novel eukaryotic magnesium-dependent phosphatase, Biochemistry, 39, 8315 (2000).
87. Shisheva, A., Rusin, B., Ikonomov, O. C., DeMarco, C., and Sbrissa, D., Localization and insulin-regulated relocation of phosphoinositide 5- kinase PIKfyve in 3T3-L1 adipocytes, J Biol Chem, 276, 11859 (2001).
88. Sims, B., Mahnke-Zizelman, D. K., Profit, A. A., Prestwich, G. D., Sabina, R. L., and Theibert, A. B., Regulation of AMP deaminase by phosphoinositides, J Biol Chem, 274, 25701 (1999).
89. Snyder, J. T., Rossman, K. L., Baumeister, M. A., Pruitt, W. M., Siderovski, D. P., Der, C. J., Lemmon, M. A., and Sondek, J., Quantitative analysis of the effect of phosphoinositide interactions on the function of Dbl family proteins, J Biol Chem, 276, 45868 (2001).
90. Song, X., Xu, W., Zhang, A., Huang, G., Liang, X., Virbasius, J. V., Czech, M. P., and Zhou, G. W., Phox homology domains specifically bind phosphatidylinositol phosphates, Biochemistry, 40, 8940 (2001).
91. Soughayer, J. S., Wang, Y., Li, H., Cheung, S. H., Rossi, F. M., Stanbridge, E. J., Sims, C. E., and Allbritton, N. L., Characterization of TAT-mediated transport of detachable kinase substrates, Biochemistry, 43, 8528 (2004).
92. Stratford, S., Hoehn, K. L., Liu, F., and Summers, S. A., Regulation of Insulin Action by Ceramide: DUAL MECHANISMS LINKING CERAMIDE ACCUMULATION TO THE INHIBITION OF Akt/PROTEIN KINASE B, J Biol Chem, 279, 36608 (2004).
93. Sumitomo, M., Iwase, A., Zheng, R., Navarro, D., Kaminetzky, D., Shen, R., Georgescu, M. M., and Nanus, D. M., Synergy in tumor suppression by direct interaction of neutral endopeptidase with PTEN, Cancer Cell, 5, 67 (2004).
94. Sweeney, G., Garg, R. R., Ceddia, R. B., Li, D., Ishiki, M., Somwar, R., Foster, L. J., Neilsen, P. O., Prestwich, G. D., Rudich, A., and Klip, A., Intracellular delivery of phosphatidylinositol (3,4,5)-trisphosphate causes incorporation of glucose transporter 4 into the plasma membrane of muscle and fat cells without increasing glucose uptake, J Biol Chem, 279, 32233 (2004).
95. Takeuchi, H., Oike, M., Paterson, H. F., Allen, V., Kanematsu, T., Ito, Y., Erneux, C., Katan, M., and Hirata, M., Inhibition of Ca2+ signalling by p130, a phospholipase-C-related catalytically inactive protein: critical role of the p130 pleckstrin homology domain, Biochem J, 349, 357 (2000).
96. Taylor, G. S., Maehama, T., and Dixon, J. E., Inaugural article: myotubularin, a protein tyrosine phosphatase mutated in myotubular myopathy, dephosphorylates the lipid second messenger, phosphatidylinositol 3-phosphate, Proc Natl Acad Sci U S A, 97, 8910 (2000).
97. Thapar, R., Karnoub, A. E., and Campbell, S. L., Structural and biophysical insights into the role of the insert region in Rac1 function, Biochemistry, 41, 3875 (2002).
98. Thomas, C. C., Dowler, S., Deak, M., Alessi, D. R., and Van Aalten, D. M., Crystal structure of the phosphatidylinositol 3,4-bisphosphate-binding pleckstrin homology (PH) domain of tandem PH-domain-containing protein 1 (TAPP1): molecular basis of lipid specificity, Biochem J, 358, 287 (2001).
99. Tong, Q., Gamper, N., Medina, J. L., Shapiro, M. S., and Stockand, J. D., Direct activation of the epithelial Na(+) channel by phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate produced by phosphoinositide 3-OH kinase, J Biol Chem, 279, 22654 (2004).
100.Virbasius, J. V., Song, X., Pomerleau, D. P., Zhan, Y., Zhou, G. W., and Czech, M. P., Activation of the Akt-related cytokine-independent survival kinase requires interaction of its phox domain with endosomal phosphatidylinositol 3-phosphate, Proc Natl Acad Sci U S A, 98, 12908 (2001).
101.Ward, D. M., Shiflett, S. L., Huynh, D., Vaughn, M. B., Prestwich, G., and Kaplan, J., Use of Expression Constructs to Dissect the Functional Domains of the CHS/Beige Protein: Identification of Multiple Phenotypes, Traffic, 4, 403 (2003).
102.Weiner, O. D., Neilsen, P. O., Prestwich, G. D., Kirschner, M. W., Cantley, L. C., and Bourne, H. R., A PtdInsP(3)- and Rho GTPase-mediated positive feedback loop regulates neutrophil polarity, Nat Cell Biol, 4, 509 (2002).
103.Wu, Y., Dowbenko, D., Pisabarro, M. T., Dillard-Telm, L., Koeppen, H., and Lasky, L. A., Pten 2, a golgi-associated testis-specific homologue of the pten tumor suppressor lipid phosphatase, J Biol Chem, 276, 21745 (2001).
104.Xiang Sun, C., Downey, G. P., Zhu, F., Koh, A. L., Thang, H., and Glogauer, M., Rac1 is the small GTPase Responsible for Regulating the Neutrophil Chemotaxis Compass, Blood, 2004 (2004).
105.Xing, Y., Liu, D., Zhang, R., Joachimiak, A., Songyang, Z., and Xu, W., Structural basis of membrane targeting by the Phox homology domain of cytokine-independent survival kinase (CISK-PX), J Biol Chem, 279, 30662 (2004).
106.Zhang, X., Loijens, J. C., Boronenkov, I. V., Parker, G. J., Norris, F. A., Chen, J., Thum, O., Prestwich, G. D., Majerus, P. W., and Anderson, R. A., Phosphatidylinositol-4-phosphate 5-kinase isozymes catalyze the synthesis of 3-phosphate-containing phosphatidylinositol signaling molecules, J Biol Chem, 272, 17756 (1997).
107.Zhu, H., Bilgin, M., Bangham, R., Hall, D., Casamayor, A., Bertone, P., Lan, N., Jansen, R., Bidlingmaier, S., Houfek, T., Mitchell, T., Miller, P., Dean, R. A., Gerstein, M., and Snyder, M., Global analysis of protein activities using proteome chips, Science, 293, 2101 (2001).
108.Zimmermann, P., Meerschaert, K., Reekmans, G., Leenaerts, I., Small, J. V., Vandekerckhove, J., David, G., and Gettemans, J., PIP(2)-PDZ domain binding controls the association of syntenin with the plasma membrane, Mol Cell, 9, 1215 (2002).

All 4'PIPs (P-4###)

1. Ahn, J.-Y., Rong, R., Liu, X., and Ye, K., PIKE/nuclear PI 3-kinase signaling mediates the antiapoptotic actions of NGF in the nucleus, EMBO J., sj.emboj.7600392 (2004).
2. Balla, A., Tuymetova, G., Barshishat, M., Geiszt, M., and Balla, T., Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments, J Biol Chem, 277, 20041 (2002).
3. Barylko, B., Gerber, S. H., Binns, D. D., Grichine, N., Khvotchev, M., Sudhof, T. C., and Albanesi, J. P., A Novel Family of Phosphatidylinositol 4-Kinases Conserved from Yeast to Humans, J Biol Chem, 276, 7705 (2001).
4. Blader, I. J., Cope, M. J., Jackson, T. R., Profit, A. A., Greenwood, A. F., Drubin, D. G., Prestwich, G. D., and Theibert, A. B., GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro, Mol Biol Cell, 10, 581 (1999).
5. Campbell, R. B., Liu, F., and Ross, A. H., Allosteric activation of PTEN phosphatase by phosphatidylinositol 4,5-bisphosphate, J Biol Chem, 278, 33617 (2003).
6. Chang, J. D., Field, S. J., Rameh, L. E., Carpenter, C. L., and Cantley, L. C., Identification and characterization of a phosphoinositide phosphate kinase homolog, J Biol Chem, 279, 11672 (2004).
7. Cheever, M. L., Sato, T. K., de Beer, T., Kutateladze, T. G., Emr, S. D., and Overduin, M., Phox domain interaction with PtdIns(3)P targets the Vam7 t-SNARE to vacuole membranes, Nat Cell Biol, 3, 613 (2001).
8. Chen, R., Kang, V. H., Chen, J., Shope, J. C., Torabinejad, J., DeWald, D. B., and Prestwich, G. D., A monoclonal antibody to visualize PtdIns(3,4,5)P(3) in cells, J Histochem Cytochem, 50, 697 (2002).
9. Chen, G., Raman, P., Bhonagiri, P., Strawbridge, A. B., Pattar, G. R., and Elmendorf, J. S., Protective Effect of Phosphatidylinositol 4,5-Bisphosphate against Cortical Filamentous Actin Loss and Insulin Resistance Induced by Sustained Exposure of 3T3-L1 Adipocytes to Insulin, J. Biol. Chem., 279, 39705 (2004).
10. Chi, Y., Zhou, B., Wang, W.-Q., Chung, S.-K., Kwon, Y.-U., Ahn, Y.-H., Chang, Y.-T., Tsujishita, Y., Hurley, J. H., and Zhang, Z.-Y., Comparative mechanistic and substrate specificity study of inositol polyphosphate 5-phosphatases SPsynaptojanin and SHIP2, J. Biol. Chem., M406416200 (2004).
11. Clarke, J. H., Letcher, A. J., D'Santos C, S., Halstead, J. R., Irvine, R. F., and Divecha, N., Inositol lipids are regulated during cell cycle progression in the nuclei of murine erythroleukaemia cells, Biochem J, 357, 905 (2001).
12. Clarke, J. H., Lipid signalling: picking out the PIPs, Curr Biol, 13, R815 (2003).
13. Corgan, A. M., Singleton, C., Santoso, C. B., and Greenwood, J. A., Phosphoinositides differentially regulate alpha-actinin flexibility and function, Biochem J, 378, 1067 (2004).
14. Dove, S. K., Piper, R.C., McEwen, R.K., Yu, J.W., King, M.C., Hughes, D.C., Thuring, J., Holmes, A.B., Cooke, F.T., Michell, R.H., Parker, P.J., & Lemmon, M.A., Svp1p defines a family of phosphatidylinositol 3,5-bisphosphate effectors, The EMBO Journal, 1 (2004).
15. Dowler, S., Currie, R. A., Downes, C. P., and Alessi, D. R., DAPP1: a dual adaptor for phosphotyrosine and 3-phosphoinositides, Biochem J, 342, 7 (1999).
16. Du, X., Zhang, H., Lopes, C., Mirshahi, T., Rohacs, T., and Logothetis, D. E., Characteristic interactions with phosphatidylinositol 4,5-bisphosphate determine regulation of kir channels by diverse modulators, J Biol Chem, 279, 37271 (2004).
17. Elge, S., Brearley, C., Xia, H. J., Kehr, J., Xue, H. W., and Mueller-Roeber, B., An Arabidopsis inositol phospholipid kinase strongly expressed in procambial cells: synthesis of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 in insect cells by 5-phosphorylation of precursors, Plant J, 26, 561 (2001).
18. Fleming, I. N., Gray, A., and Downes, C. P., Regulation of the Rac1-specific exchange factor Tiam1 involves both phosphoinositide 3-kinase-dependent and -independent components, Biochem J, 351, 173 (2000).
19. Fraley, T. S., Tran, T. C., Corgan, A. M., Nash, C. A., Hao, J., Critchley, D. R., and Greenwood, J. A., Phosphoinositide binding inhibits alpha -actinin bundling activity, J Biol Chem, 24, 24 (2003).
20. Friant, S., Pecheur, E. I., Eugster, A., Michel, F., Lefkir, Y., Nourrisson, D., and Letourneur, F., Ent3p Is a PtdIns(3,5)P2 effector required for protein sorting to the multivesicular body, Dev Cell, 5, 499 (2003).
21. Fuster, D., Moe, O. W., and Hilgemann, D. W., Lipid- and mechanosensitivities of sodium/hydrogen exchangers analyzed by electrical methods, Proc Natl Acad Sci U S A, 101, 10482 (2004).
22. Gaidarov, I., Krupnick, J. G., Falck, J. R., Benovic, J. L., and Keen, J. H., Arrestin function in G protein-coupled receptor endocytosis requires phosphoinositide binding, Embo J, 18, 871 (1999).
23. Gozani, O., Karuman, P., Jones, D. R., Ivanov, D., Cha, J., Lugovskoy, A. A., Baird, C. L., Zhu, H., Field, S. J., Lessnick, S. L., Villasenor, J., Mehrotra, B., Chen, J., Rao, V. R., Brugge, J. S., Ferguson, C. G., Payrastre, B., Myszka, D. G., Cantley, L. C., Wagner, G., Divecha, N., Prestwich, G. D., and Yuan, J., The PHD finger of the chromatin-associated protein ING2 functions as a nuclear phosphoinositide receptor, Cell, 114, 99 (2003).
24. Guo, J., Wenk, M. R., Pellegrini, L., Onofri, F., Benfenati, F., and De Camilli, P., Phosphatidylinositol 4-kinase type IIalpha is responsible for the phosphatidylinositol 4-kinase activity associated with synaptic vesicles, Proc Natl Acad Sci U S A, 100, 3995 (2003).
25. Hama, H., Torabinejad, J., Prestwich, G. D., and DeWald, D. B., Measurement and Immunofluorescence of Cellular Phosphoinositides, in Methods in Molecular Biology: Signal Transduction Protocols, Vol. 284, Dickson, R. C., Ed., Humana Press Inc., Totowa, NJ, pp. 243 (2003).
26. Heras, B., and Drobak, B. K., PARF-1: an Arabidopsis thaliana FYVE-domain protein displaying a novel eukaryotic domain structure and phosphoinositide affinity, J Exp Bot, 53, 565 (2002).
27. Hinchliffe, K. A., Giudici, M. L., Letcher, A. J., and Irvine, R. F., Type IIalpha phosphatidylinositol phosphate kinase associates with the plasma membrane via interaction with type I isoforms, Biochem J, 363, 563 (2002).
28. Hirdes, W., Horowitz, L. F., and Hille, B., Muscarinic modulation of erg potassium current, J. Physiol., 559, 67 (2004).
29. Hodgkin, M. N., Masson, M. R., Powner, D., Saqib, K. M., Ponting, C. P., and Wakelam, M. J., Phospholipase D regulation and localisation is dependent upon a phosphatidylinositol 4,5-biphosphate-specific PH domain, Curr Biol, 10, 43 (2000).
30. Huang, Z., Guo, X. X., Chen, S. X., Alvarez, K. M., Bell, M. W., and Anderson, R. E., Regulation of Type II Phosphatidylinositol Phosphate Kinase by Tyrosine Phosphorylation in Bovine Rod Outer Segments, Biochemistry, 40, 4550 (2001).
31. Iijima, M., Huang, Y. E., Luo, H. R., Vazquez, F., and Devreotes, P. N., Novel Mechanism of PTEN Regulation by Its Phosphatidylinositol 4,5-Bisphosphate Binding Motif Is Critical for Chemotaxis, J Biol Chem, 279, 16606 (2004).
32. Ikonomov, O. C., Sbrissa, D., Mlak, K., Kanzaki, M., Pessin, J., and Shisheva, A., Functional dissection of lipid and protein kinase signals of PIKfyve reveals the role of PtdIns 3,5-P2 production for endomembrane integrity, J Biol Chem, 277, 9206 (2002).
33. Jenco, J. M., Rawlingson, A., Daniels, B., and Morris, A. J., Regulation of phospholipase D2: selective inhibition of mammalian phospholipase D isoenzymes by alpha- and beta-synucleins, Biochemistry, 37, 4901 (1998).
34. Johnson, L. M., James, K. M., Chamberlain, M. D., and Anderson, D. H., Identification of Key Residues in the A-Raf Kinase Important for Phosphoinositide Lipid Binding Specificity, Biochemistry, 44, 3432 (2005).
35. Kalthoff, C., Groos, S., Kohl, R., Mahrhold, S., and Ungewickell, E. J., Clint: a novel clathrin-binding ENTH-domain protein at the Golgi, Mol Biol Cell, 13, 4060 (2002).
36. Kearns, M. A., Monks, D. E., Fang, M., Rivas, M. P., Courtney, P. D., Chen, J., Prestwich, G. D., Theibert, A. B., Dewey, R. E., and Bankaitis, V. A., Novel developmentally regulated phosphoinositide binding proteins from soybean whose expression bypasses the requirement for an essential phosphatidylinositol transfer protein in yeast, Embo J, 17, 4004 (1998).
37. Kim, S. A., Taylor, G. S., Torgersen, K. M., and Dixon, J. E., Myotubularin and MTMR2, phosphatidylinositol 3-phosphatases mutated in myotubular myopathy and type 4B Charcot-Marie-Tooth disease, J Biol Chem, 277, 4526 (2002).
38. Kunz, J., Fuelling, A., Kolbe, L., and Anderson, R. A., Stereo-specific substrate recognition by phosphatidylinositol phosphate kinases is swapped by changing a single amino acid residue, J Biol Chem, 277, 5611 (2002).
39. Le Blanc, C., Mironneau, C., Barbot, C., Henaff, M., Bondeva, T., Wetzker, R., and Macrez, N., Regulation of Vascular L-type Ca2+ Channels by Phosphatidylinositol 3,4,5-Trisphosphate, Circ. Res., 95, 300 (2004).
40. Lee, S. J., and Montell, C., Light-dependent translocation of visual arrestin regulated by the NINAC myosin III, Neuron, 43, 95 (2004).
41. Liu, D., and Liman, E. R., Intracellular Ca2+ and the phospholipid PIP2 regulate the taste transduction ion channel TRPM5, Proc Natl Acad Sci U S A, 100, 15160 (2003).
42. Macia, E., Paris, S., and Chabre, M., Binding of the PH and polybasic C-terminal domains of ARNO to phosphoinositides and to acidic lipids, Biochemistry, 39, 5893 (2000).
43. Maffucci, T., Razzini, G., Ingrosso, A., Chen, H., Iacobelli, S., Sciacchitano, S., Quon, M. J., and Falasca, M., Role of pleckstrin homology domain in regulating membrane targeting and metabolic function of insulin receptor substrate 3, Mol Endocrinol, 17, 1568 (2003).
44. Marcus, S. L., Wenk, M. R., Steele-Mortimer, O., and Finlay, B. B., A synaptojanin-homologous region of Salmonella typhimurium SigD is essential for inositol phosphatase activity and Akt activation, FEBS Lett, 494, 201 (2001).
45. Mehrotra, B., Myszka, D. G., and Prestwich, G. D., Binding kinetics and ligand specificity for the interactions of the C2B domain of synaptogmin II with inositol polyphosphates and phosphoinositides, Biochemistry, 39, 9679 (2000).
46. Naga Prasad, S. V., Laporte, S. A., Chamberlain, D., Caron, M. G., Barak, L., and Rockman, H. A., Phosphoinositide 3-kinase regulates beta2-adrenergic receptor endocytosis by AP-2 recruitment to the receptor/beta-arrestin complex, J Cell Biol, 158, 563 (2002).
47. Oganesian, A., Poot, M., Daum, G., Coats, S. A., Wright, M. B., Seifert, R. A., and Bowen-Pope, D. F., Protein tyrosine phosphatase RQ is a phosphatidylinositol phosphatase that can regulate cell survival and proliferation, Proc Natl Acad Sci U S A, 100, 7563 (2003).
48. Ortiz, P. A., Hong, N. J., and Garvin, J. L., Luminal flow induces eNOS activation and translocation in the rat thick ascending limb. II. Role of PI3-kinase and Hsp90, Am J Physiol Renal Physiol, 287, F281 (2004).
49. Osborne, S. L., Thomas, C. L., Gschmeissner, S., and Schiavo, G., Nuclear PtdIns(4,5)P2 assembles in a mitotically regulated particle involved in pre-mRNA splicing, J Cell Sci, 114, 2501 (2001).
50. Ozaki, S., DeWald, D. B., Shope, J. C., Chen, J., and Prestwich, G. D., Intracellular delivery of phosphoinositides and inositol phosphates using polyamine carriers, Proc Natl Acad Sci U S A, 97, 11286 (2000).
51. Peterman, T. K., Ohol, Y. M., McReynolds, L. J., and Luna, E. J., Patellin1, a Novel Sec14-Like Protein, Localizes to the Cell Plate and Binds Phosphoinositides, Plant Physiology, pp.104.045369 (2004).
52. Rajala, R. V., and Anderson, R. E., Interaction of the insulin receptor beta-subunit with phosphatidylinositol 3-kinase in bovine ROS, Invest Ophthalmol Vis Sci, 42, 3110 (2001).
53. Rajala, R. V., McClellan, M. E., Ash, J. D., and Anderson, R. E., In vivo regulation of phosphoinositide 3-kinase in retina through light- induced tyrosine phosphorylation of the insulin receptor beta-subunit, J Biol Chem, 277, 43319 (2002).
54. Rajala, R. V., McClellan, M. E., Chan, M. D., Tsiokas, L., and Anderson, R. E., Interaction of the retinal insulin receptor beta-subunit with the P85 subunit of phosphoinositide 3-kinase, Biochemistry, 43, 5637 (2004).
55. Rao, V. R., Corradetti, M. N., Chen, J., Peng, J., Yuan, J., Prestwich, G. D., and Brugge, J. S., Expression cloning of protein targets for 3-phosphorylated phosphoinositides, J Biol Chem, 274, 37893 (1999).
56. Razzini, G., Ingrosso, A., Brancaccio, A., Sciacchitano, S., Esposito, D. L., and Falasca, M., Different subcellular localization and phosphoinositides binding of insulin receptor substrate protein pleckstrin homology domains, Mol Endocrinol, 14, 823 (2000).
57. Redfern, D. A., and Gericke, A., Domain formation in phosphatidylinositol monophosphate/phosphatidylcholine mixed vesicles, Biophys J, 86, 2980 (2004).
58. Rohacs, T., Chen, J., Prestwich, G. D., and Logothetis, D. E., Distinct specificities of inwardly rectifying K(+) channels for phosphoinositides, J Biol Chem, 274, 36065 (1999).
59. Rohacs, T., Lopes, C., Mirshahi, T., Jin, T., Zhang, H., and Logothetis, D. E., Assaying phosphatidylinositol bisphosphate regulation of potassium channels, Methods Enzymol, 345, 71 (2002).
60. Rohacs, T., Lopes, C. M., Jin, T., Ramdya, P. P., Molnar, Z., and Logothetis, D. E., Specificity of activation by phosphoinositides determines lipid regulation of Kir channels, Proc Natl Acad Sci U S A, 100, 745 (2003).
61. Rossman, K. L., Cheng, L., Mahon, G. M., Rojas, R. J., Snyder, J. T., Whitehead, I. P., and Sondek, J., Multifunctional roles for the PH domain of Dbs in regulating Rho GTPase activation, J Biol Chem, 278, 18393 (2003).
62. Runnels, L. W., Yue, L., and Clapham, D. E., The TRPM7 channel is inactivated by PIP(2) hydrolysis, Nat Cell Biol, 4, 329 (2002).
63. Russo, C., Gao, Y., Mancini, P., Vanni, C., Porotto, M., Falasca, M., Torrisi, M. R., Zheng, Y., and Eva, A., Modulation of oncogenic DBL activity by phosphoinositol phosphate binding to pleckstrin homology domain, J Biol Chem, 276, 19524 (2001).
64. Rzepecki, P. W., and Prestwich, G. D., Synthesis of hybrid lipid probes: derivatives of phosphatidylethanolamine-extended phosphatidylinositol 4,5-bisphosphate (Pea-PIP(2)), J Org Chem, 67, 5454 (2002).
65. Sbrissa, D., Ikonomov, O. C., and Shisheva, A., PIKfyve, a mammalian ortholog of yeast Fab1p lipid kinase, synthesizes 5-phosphoinositides. Effect of insulin, J Biol Chem, 274, 21589 (1999).
66. Seidel, R. D., 3rd, Amor, J. C., Kahn, R. A., and Prestegard, J. H., Structural perturbations in human ADP ribosylation factor-1 accompanying the binding of phosphatidylinositides, Biochemistry, 43, 15393 (2004).
67. Selengut, J. D., and Levine, R. L., MDP-1: A novel eukaryotic magnesium-dependent phosphatase, Biochemistry, 39, 8315 (2000).
68. Snyder, J. T., Rossman, K. L., Baumeister, M. A., Pruitt, W. M., Siderovski, D. P., Der, C. J., Lemmon, M. A., and Sondek, J., Quantitative analysis of the effect of phosphoinositide interactions on the function of Dbl family proteins, J Biol Chem, 276, 45868 (2001).
69. Song, X., Xu, W., Zhang, A., Huang, G., Liang, X., Virbasius, J. V., Czech, M. P., and Zhou, G. W., Phox homology domains specifically bind phosphatidylinositol phosphates, Biochemistry, 40, 8940 (2001).
70. Takeuchi, H., Oike, M., Paterson, H. F., Allen, V., Kanematsu, T., Ito, Y., Erneux, C., Katan, M., and Hirata, M., Inhibition of Ca2+ signalling by p130, a phospholipase-C-related catalytically inactive protein: critical role of the p130 pleckstrin homology domain, Biochem J, 349, 357 (2000).
71. Taylor, G. S., Maehama, T., and Dixon, J. E., Inaugural article: myotubularin, a protein tyrosine phosphatase mutated in myotubular myopathy, dephosphorylates the lipid second messenger, phosphatidylinositol 3-phosphate, Proc Natl Acad Sci U S A, 97, 8910 (2000).
72. Thapar, R., Karnoub, A. E., and Campbell, S. L., Structural and biophysical insights into the role of the insert region in Rac1 function, Biochemistry, 41, 3875 (2002).
73. Tong, Q., Gamper, N., Medina, J. L., Shapiro, M. S., and Stockand, J. D., Direct activation of the epithelial Na(+) channel by phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate produced by phosphoinositide 3-OH kinase, J Biol Chem, 279, 22654 (2004).
74. Varnai, P., Lin, X., Lee, S. B., Tuymetova, G., Bondeva, T., Spat, A., Rhee, S. G., Hajnoczky, G., and Balla, T., Inositol lipid binding and membrane localization of isolated pleckstrin homology (PH) domains. Studies on the PH domains of phospholipase C delta 1 and p130, J Biol Chem, 277, 27412 (2002).
75. Virbasius, J. V., Song, X., Pomerleau, D. P., Zhan, Y., Zhou, G. W., and Czech, M. P., Activation of the Akt-related cytokine-independent survival kinase requires interaction of its phox domain with endosomal phosphatidylinositol 3-phosphate, Proc Natl Acad Sci U S A, 98, 12908 (2001).
76. Wang, P. Y., Kitchens, R. L., and Munford, R. S., Phosphatidylinositides bind to plasma membrane CD14 and can prevent monocyte activation by bacterial lipopolysaccharide, J Biol Chem, 273, 24309 (1998).
77. Ward, D. M., Shiflett, S. L., Huynh, D., Vaughn, M. B., Prestwich, G., and Kaplan, J., Use of Expression Constructs to Dissect the Functional Domains of the CHS/Beige Protein: Identification of Multiple Phenotypes, Traffic, 4, 403 (2003).
78. Yu, J. W., and Lemmon, M. A., All phox homology (PX) domains from Saccharomyces cerevisiae specifically recognize phosphatidylinositol 3-phosphate, J Biol Chem, 276, 44179 (2001).
79. Yun, M., Keshvara, L., Park, C. G., Zhang, Y. M., Dickerson, J. B., Zheng, J., Rock, C. O., Curran, T., and Park, H. W., Crystal structures of the Dab homology domains of mouse disabled 1 and 2, J Biol Chem, 278, 36572 (2003).
80. Zhu, H., Bilgin, M., Bangham, R., Hall, D., Casamayor, A., Bertone, P., Lan, N., Jansen, R., Bidlingmaier, S., Houfek, T., Mitchell, T., Miller, P., Dean, R. A., Gerstein, M., and Snyder, M., Global analysis of protein activities using proteome chips, Science, 293, 2101 (2001).
81. Zimmermann, P., Meerschaert, K., Reekmans, G., Leenaerts, I., Small, J. V., Vandekerckhove, J., David, G., and Gettemans, J., PIP(2)-PDZ domain binding controls the association of syntenin with the plasma membrane, Mol Cell, 9, 1215 (2002).

All 5'PIPs (P-5###)

1. Arneson, L. S., Kunz, J., Anderson, R. A., and Traub, L. M., Coupled inositide phosphorylation and phospholipase D activation initiates clathrin-coat assembly on lysosomes, J Biol Chem, 274, 17794 (1999).
2. Barylko, B., Gerber, S. H., Binns, D. D., Grichine, N., Khvotchev, M., Sudhof, T. C., and Albanesi, J. P., A Novel Family of Phosphatidylinositol 4-Kinases Conserved from Yeast to Humans, J Biol Chem, 276, 7705 (2001).
3. Burden, L. M., Rao, V. D., Murray, D., Ghirlando, R., Doughman, S. D., Anderson, R. A., and Hurley, J. H., The flattened face of type II beta phosphatidylinositol phosphate kinase binds acidic phospholipid membranes, Biochemistry, 38, 15141 (1999).
4. Chang, J. D., Field, S. J., Rameh, L. E., Carpenter, C. L., and Cantley, L. C., Identification and characterization of a phosphoinositide phosphate kinase homolog, J Biol Chem, 279, 11672 (2004).
5. Chi, Y., Zhou, B., Wang, W.-Q., Chung, S.-K., Kwon, Y.-U., Ahn, Y.-H., Chang, Y.-T., Tsujishita, Y., Hurley, J. H., and Zhang, Z.-Y., Comparative mechanistic and substrate specificity study of inositol polyphosphate 5-phosphatases SPsynaptojanin and SHIP2, J. Biol. Chem., M406416200 (2004).
6. Clarke, J. H., Letcher, A. J., D'Santos C, S., Halstead, J. R., Irvine, R. F., and Divecha, N., Inositol lipids are regulated during cell cycle progression in the nuclei of murine erythroleukaemia cells, Biochem J, 357, 905 (2001).
7. Clarke, J. H., Lipid signalling: picking out the PIPs, Curr Biol, 13, R815 (2003).
8. Coppolino, M. G., Dierckman, R., Loijens, J., Collins, R. F., Pouladi, M., Jongstra-Bilen, J., Schreiber, A. D., Trimble, W. S., Anderson, R., and Grinstein, S., Inhibition of phosphatidylinositol-4-phosphate 5-kinase Ialpha impairs localized actin remodeling and suppresses phagocytosis, J Biol Chem, 277, 43849 (2002).
9. Crottet, P., Meyer, D. M., Rohrer, J., and Spiess, M., ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5- bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes, Mol Biol Cell, 13, 3672 (2002).
10. Davis, A. J., Perera, I. Y., and Boss, W. F., Cyclodextrins enhance recombinant phosphatidylinositol phosphate kinase activity, J. Lipid Res., 45, 1783 (2004).
11. Dove, S. K., Piper, R.C., McEwen, R.K., Yu, J.W., King, M.C., Hughes, D.C., Thuring, J., Holmes, A.B., Cooke, F.T., Michell, R.H., Parker, P.J., & Lemmon, M.A., Svp1p defines a family of phosphatidylinositol 3,5-bisphosphate effectors, The EMBO Journal, 1 (2004).
12. Guo, J., Wenk, M. R., Pellegrini, L., Onofri, F., Benfenati, F., and De Camilli, P., Phosphatidylinositol 4-kinase type IIalpha is responsible for the phosphatidylinositol 4-kinase activity associated with synaptic vesicles, Proc Natl Acad Sci U S A, 100, 3995 (2003).
13. Hama, H., Torabinejad, J., Prestwich, G. D., and DeWald, D. B., Measurement and Immunofluorescence of Cellular Phosphoinositides, in Methods in Molecular Biology: Signal Transduction Protocols, Vol. 284, Dickson, R. C., Ed., Humana Press Inc., Totowa, NJ, pp. 243 (2003).
14. He, F., Mao, M., and Wensel, T. G., Enhancement of phototransduction g protein-effector interactions by phosphoinositides, J Biol Chem, 279, 8986 (2004).
15. Heras, B., and Drobak, B. K., PARF-1: an Arabidopsis thaliana FYVE-domain protein displaying a novel eukaryotic domain structure and phosphoinositide affinity, J Exp Bot, 53, 565 (2002).
16. Hinchliffe, K. A., Giudici, M. L., Letcher, A. J., and Irvine, R. F., Type IIalpha phosphatidylinositol phosphate kinase associates with the plasma membrane via interaction with type I isoforms, Biochem J, 363, 563 (2002).
17. Huang, Z., Guo, X. X., Chen, S. X., Alvarez, K. M., Bell, M. W., and Anderson, R. E., Regulation of Type II Phosphatidylinositol Phosphate Kinase by Tyrosine Phosphorylation in Bovine Rod Outer Segments, Biochemistry, 40, 4550 (2001).
18. Ikonomov, O. C., Sbrissa, D., Mlak, K., Kanzaki, M., Pessin, J., and Shisheva, A., Functional dissection of lipid and protein kinase signals of PIKfyve reveals the role of PtdIns 3,5-P2 production for endomembrane integrity, J Biol Chem, 277, 9206 (2002).
19. Johnson, L. M., James, K. M., Chamberlain, M. D., and Anderson, D. H., Identification of Key Residues in the A-Raf Kinase Important for Phosphoinositide Lipid Binding Specificity, Biochemistry, 44, 3432 (2005).
20. Kalthoff, C., Groos, S., Kohl, R., Mahrhold, S., and Ungewickell, E. J., Clint: a novel clathrin-binding ENTH-domain protein at the Golgi, Mol Biol Cell, 13, 4060 (2002).
21. Kim, S. A., Taylor, G. S., Torgersen, K. M., and Dixon, J. E., Myotubularin and MTMR2, phosphatidylinositol 3-phosphatases mutated in myotubular myopathy and type 4B Charcot-Marie-Tooth disease, J Biol Chem, 277, 4526 (2002).
22. Kruger, J. M., Fukushima, T., Cherepanov, V., Borregaard, N., Loeve, C., Shek, C., Sharma, K., Tanswell, A. K., Chow, C. W., and Downey, G. P., Protein-tyrosine phosphatase MEG2 is expressed by human neutrophils. Localization to the phagosome and activation by polyphosphoinositides, J Biol Chem, 277, 2620 (2002).
23. Kunz, J., Wilson, M. P., Kisseleva, M., Hurley, J. H., Majerus, P. W., and Anderson, R. A., The activation loop of phosphatidylinositol phosphate kinases determines signaling specificity, Mol Cell, 5, 1 (2000).
24. Kunz, J., Fuelling, A., Kolbe, L., and Anderson, R. A., Stereo-specific substrate recognition by phosphatidylinositol phosphate kinases is swapped by changing a single amino acid residue, J Biol Chem, 277, 5611 (2002).
25. Kutateladze, T. G., Ogburn, K. D., Watson, W. T., de Beer, T., Emr, S. D., Burd, C. G., and Overduin, M., Phosphatidylinositol 3-phosphate recognition by the FYVE domain, Mol Cell, 3, 805 (1999).
26. Marcus, S. L., Wenk, M. R., Steele-Mortimer, O., and Finlay, B. B., A synaptojanin-homologous region of Salmonella typhimurium SigD is essential for inositol phosphatase activity and Akt activation, FEBS Lett, 494, 201 (2001).
26. Marcus, S. L., Wenk, M. R., Steele-Mortimer, O., and Finlay, B. B., A synaptojanin-homologous region of Salmonella typhimurium SigD is essential for inositol phosphatase activity and Akt activation, FEBS Lett, 494, 201 (2001).
27. Oganesian, A., Poot, M., Daum, G., Coats, S. A., Wright, M. B., Seifert, R. A., and Bowen-Pope, D. F., Protein tyrosine phosphatase RQ is a phosphatidylinositol phosphatase that can regulate cell survival and proliferation, Proc Natl Acad Sci U S A, 100, 7563 (2003).
28. Ooms, L. M., McColl, B. K., Wiradjaja, F., Wijayaratnam, A. P., Gleeson, P., Gething, M. J., Sambrook, J., and Mitchell, C. A., The yeast inositol polyphosphate 5-phosphatases inp52p and inp53p translocate to actin patches following hyperosmotic stress: mechanism for regulating phosphatidylinositol 4,5-bisphosphate at plasma membrane invaginations, Mol Cell Biol, 20, 9376 (2000).
29. Peterman, T. K., Ohol, Y. M., McReynolds, L. J., and Luna, E. J., Patellin1, a Novel Sec14-Like Protein, Localizes to the Cell Plate and Binds Phosphoinositides, Plant Physiology, pp.104.045369 (2004).
30. Rao, V. D., Misra, S., Boronenkov, I. V., Anderson, R. A., and Hurley, J. H., Structure of type IIbeta phosphatidylinositol phosphate kinase: a protein kinase fold flattened for interfacial phosphorylation, Cell, 94, 829 (1998).
31. Redfern, D. A., and Gericke, A., Domain formation in phosphatidylinositol monophosphate/phosphatidylcholine mixed vesicles, Biophys J, 86, 2980 (2004).
32. Sankaran, V. G., Klein, D. E., Sachdeva, M. M., and Lemmon, M. A., High-affinity binding of a fyve domain to phosphatidylinositol 3- phosphate requires intact phospholipid but not fyve domain oligomerization, Biochemistry, 40, 8581 (2001).
33. Sbrissa, D., Ikonomov, O. C., and Shisheva, A., PIKfyve, a mammalian ortholog of yeast Fab1p lipid kinase, synthesizes 5-phosphoinositides. Effect of insulin, J Biol Chem, 274, 21589 (1999).
34. Sbrissa, D., Ikonomov, O. C., Deeb, R., and Shisheva, A., Phosphatidylinositol 5-phosphate biosynthesis is linked to PIKfyve and is involved in osmotic response pathway in mammalian cells, J Biol Chem, 277, 47276 (2002).
35. Shisheva, A., Sbrissa, D., and Ikonomov, O., Cloning, characterization, and expression of a novel Zn2+-binding FYVE finger-containing phosphoinositide kinase in insulin-sensitive cells, Mol Cell Biol, 19, 623 (1999).
36. Song, X., Xu, W., Zhang, A., Huang, G., Liang, X., Virbasius, J. V., Czech, M. P., and Zhou, G. W., Phox homology domains specifically bind phosphatidylinositol phosphates, Biochemistry, 40, 8940 (2001).
37. Taylor, G. S., Maehama, T., and Dixon, J. E., Inaugural article: myotubularin, a protein tyrosine phosphatase mutated in myotubular myopathy, dephosphorylates the lipid second messenger, phosphatidylinositol 3-phosphate, Proc Natl Acad Sci U S A, 97, 8910 (2000).
39. Westergren, T., Dove, S. K., Sommarin, M., and Pical, C., AtPIP5K1, an Arabidopsis thaliana phosphatidylinositol phosphate kinase, synthesizes PtdIns(3,4)P(2) and PtdIns(4,5)P(2) in vitro and is inhibited by phosphorylation, Biochem J, 359, 583 (2001).

Inositol Phosphates

1. Chi, Y., Zhou, B., Wang, W.-Q., Chung, S.-K., Kwon, Y.-U., Ahn, Y.-H., Chang, Y.-T., Tsujishita, Y., Hurley, J. H., and Zhang, Z.-Y., Comparative mechanistic and substrate specificity study of inositol polyphosphate 5-phosphatases SPsynaptojanin and SHIP2, J. Biol. Chem., M406416200 (2004).
2. Gaullier, J. M., Ronning, E., Gillooly, D. J., and Stenmark, H., Interaction of the EEA1 FYVE finger with phosphatidylinositol 3- phosphate and early endosomes. Role of conserved residues, J Biol Chem, 275, 24595 (2000).
3. Iijima, M., Huang, Y. E., Luo, H. R., Vazquez, F., and Devreotes, P. N., Novel Mechanism of PTEN Regulation by Its Phosphatidylinositol 4,5-Bisphosphate Binding Motif Is Critical for Chemotaxis, J Biol Chem, 279, 16606 (2004).
4. Kim, S. A., Taylor, G. S., Torgersen, K. M., and Dixon, J. E., Myotubularin and MTMR2, phosphatidylinositol 3-phosphatases mutated in myotubular myopathy and type 4B Charcot-Marie-Tooth disease, J Biol Chem, 277, 4526 (2002).
5. Razzini, G., Berrie, C. P., Vignati, S., Broggini, M., Mascetta, G., Brancaccio, A., and Falasca, M., Novel functional PI 3-kinase antagonists inhibit cell growth and tumorigenicity in human cancer cell lines, Faseb J, 14, 1179 (2000).
6. Sankaran, V. G., Klein, D. E., Sachdeva, M. M., and Lemmon, M. A., High-affinity binding of a fyve domain to phosphatidylinositol 3- phosphate requires intact phospholipid but not fyve domain oligomerization, Biochemistry, 40, 8581 (2001).
7. Stolt, P. C., Vardar, D., and Blacklow, S. C., The dual-function disabled-1 PTB domain exhibits site independence in binding phosphoinositide and peptide ligands, Biochemistry, 43, 10979 (2004).
8. Taylor, G. S., Maehama, T., and Dixon, J. E., Inaugural article: myotubularin, a protein tyrosine phosphatase mutated in myotubular myopathy, dephosphorylates the lipid second messenger, phosphatidylinositol 3-phosphate, Proc Natl Acad Sci U S A, 97, 8910 (2000).

Fluorescent and Biotinylated PIPs

1. Anzelon, A. N., Wu, H., and Rickert, R. C., Pten inactivation alters peripheral B lymphocyte fate and reconstitutes CD19 function, Nat Immunol, 4, 287 (2003).
2. Davis, A. J., Perera, I. Y., and Boss, W. F., Cyclodextrins enhance recombinant phosphatidylinositol phosphate kinase activity, J. Lipid Res., 45, 1783 (2004).
3. Denisov, G., Wanaski, S., Luan, P., Glaser, M., and McLaughlin, S., Binding of basic peptides to membranes produces lateral domains enriched in the acidic lipids phosphatidylserine and phosphatidylinositol 4,5-bisphosphate: an electrostatic model and experimental results, Biophys J, 74, 731 (1998).
4. Ercetin, M. E., and Gillaspy, G. E., Molecular characterization of an Arabidopsis gene encoding a phospholipid-specific inositol polyphosphate 5-phosphatase, Plant Physiol, 135, 938 (2004).
5. Gambhir, A., Hangyas-Mihalyne, G., Zaitseva, I., Cafiso, D. S., Wang, J., Murray, D., Pentyala, S. N., Smith, S. O., and McLaughlin, S., Electrostatic Sequestration of PIP(2) on Phospholipid Membranes by Basic/Aromatic Regions of Proteins, Biophys J, 86, 2188 (2004).
6. Hairfield, M. L., Westwater, C., and Dolan, J. W., Phosphatidylinositol-4-phosphate 5-kinase activity is stimulated during temperature-induced morphogenesis in Candida albicans, Microbiology, 148, 1737 (2002).
7. Lee, S. Y., Wenk, M. R., Kim, Y., Nairn, A. C., and De Camilli, P., Regulation of synaptojanin 1 by cyclin-dependent kinase 5 at synapses, Proc Natl Acad Sci U S A, 101, 546 (2004).
8. Oganesian, A., Poot, M., Daum, G., Coats, S. A., Wright, M. B., Seifert, R. A., and Bowen-Pope, D. F., Protein tyrosine phosphatase RQ is a phosphatidylinositol phosphatase that can regulate cell survival and proliferation, Proc Natl Acad Sci U S A, 100, 7563 (2003).
9. Pagliarini, D. J., Worby, C. A., and Dixon, J. E., A PTEN-like Phosphatase with a Novel Substrate Specificity, J. Biol. Chem., 279, 38590 (2004).
10. Rudge, S. A., Anderson, D. M., and Emr, S. D., Vacuole size control: regulation of PtdIns(3,5)P2 levels by the vacuole-associated Vac14-Fig4 complex, a PtdIns(3,5)P2-specific phosphatase, Mol Biol Cell, 15, 24 (2004).
11. Rzepecki, P. W., and Prestwich, G. D., Synthesis of hybrid lipid probes: derivatives of phosphatidylethanolamine-extended phosphatidylinositol 4,5-bisphosphate (Pea-PIP(2)), J Org Chem, 67, 5454 (2002).
12. Sweeney, G., Garg, R. R., Ceddia, R. B., Li, D., Ishiki, M., Somwar, R., Foster, L. J., Neilsen, P. O., Prestwich, G. D., Rudich, A., and Klip, A., Intracellular delivery of phosphatidylinositol (3,4,5)-trisphosphate causes incorporation of glucose transporter 4 into the plasma membrane of muscle and fat cells without increasing glucose uptake, J Biol Chem, 279, 32233 (2004).
13. Taylor, G. S., and Dixon, J. E., An assay for phosphoinositide phosphatases utilizing fluorescent substrates, Anal Biochem, 295, 122 (2001).
14. Wagner, M. L., and Tamm, L. K., Reconstituted syntaxin1a/SNAP25 interacts with negatively charged lipids as measured by lateral diffusion in planar supported bilayers, Biophys J, 81, 266 (2001).
15. Wang, J. A., Anna; Hangyaw-Mihalyne, Gyongyi; McLaughlin, Stuart, The Effector Domain of Myristoylated Alanine-rich C Kinase Substrate Binds Strongly to Phosphatidylinositol 4,5-Bisphosphate, J Biol Chem, 276, 5012 (2001).

Antibodies

1. Corbalan-Garcia, S., Garcia-Garcia, J., Rodriguez-Alfaro, J. A., and Gomez-Fernandez, J. C., A new phosphatidylinositol 4,5-bisphosphate-binding site located in the C2 domain of protein kinase Calpha, J Biol Chem, 278, 4972 (2003).
2. Georgescu, M. M., Kirsch, K. H., Akagi, T., Shishido, T., and Hanafusa, H., The tumor-suppressor activity of PTEN is regulated by its carboxyl- terminal region, Proc Natl Acad Sci U S A, 96, 10182 (1999).
3. Guttman, J. A., Janmey, P., and Vogl, A. W., Gelsolin--evidence for a role in turnover of junction-related actin filaments in Sertoli cells, J Cell Sci, 115, 499 (2002).
4. Hama, H., Torabinejad, J., Prestwich, G. D., and DeWald, D. B., Measurement and Immunofluorescence of Cellular Phosphoinositides, in Methods in Molecular Biology: Signal Transduction Protocols, Vol. 284, Dickson, R. C., Ed., Humana Press Inc., Totowa, NJ, pp. 243 (2003).
5. Hirono, M., Denis, C. S., Richardson, G. P., and Gillespie, P. G., Hair cells require phosphatidylinositol 4,5-bisphosphate for mechanical transduction and adaptation, Neuron, 44, 309 (2004).
6. Hyun, T. S., Rao, D. S., Saint-Dic, D., Michael, L. E., Kumar, P. D., Bradley, S. V., Mizukami, I. F., Oravecz-Wilson, K. I., and Ross, T. S., HIP1 and HIP1r stabilize receptor tyrosine kinases and bind 3-phosphoinositides via epsin N-terminal homology domains, J Biol Chem, 279, 14294 (2004).
7. Kitamura, T., Kitamura, Y., Nakae, J., Giordano, A., Cinti, S., Kahn, C. R., Efstratiadis, A., and Accili, D., Mosaic analysis of insulin receptor function, J Clin Invest, 113, 209 (2004).
8. Kondo, T., and Kahn, C. R., Altered insulin signaling in retinal tissue in diabetic States, J Biol Chem, 279, 37997 (2004).
9. Liu, H., Radisky, D. C., Wang, F., and Bissell, M. J., Polarity and proliferation are controlled by distinct signaling pathways downstream of PI3-kinase in breast epithelial tumor cells, J Cell Biol, 164, 603 (2004).
10. Niswender, K. D., Gallis, B., Blevins, J. E., Corson, M. A., Schwartz, M. W., and Baskin, D. G., Immunocytochemical detection of phosphatidylinositol 3-kinase activation by insulin and leptin, J Histochem Cytochem, 51, 275 (2003).
11. Niswender, K. D., Morrison, C. D., Clegg, D. J., Olson, R., Baskin, D. G., Myers, M. G., Jr., Seeley, R. J., and Schwartz, M. W., Insulin activation of phosphatidylinositol 3-kinase in the hypothalamic arcuate nucleus: a key mediator of insulin-induced anorexia, Diabetes, 52, 227 (2003).
12. Ozaki, S., DeWald, D. B., Shope, J. C., Chen, J., and Prestwich, G. D., Intracellular delivery of phosphoinositides and inositol phosphates using polyamine carriers, Proc Natl Acad Sci U S A, 97, 11286 (2000).
13. Rickert, P., Weiner, O. D., Wang, F., Bourne, H. R., and Servant, G., Leukocytes navigate by compass: roles of PI3Kgamma and its lipid products, Trends Cell Biol, 10, 466 (2000).
14. Rousset, M., Cens, T., Gouin-Charnet, A., Scamps, F., and Charnet, P., Ca2+ and phosphatidylinositol 4,5-bisphosphate stabilize a Gbeta gamma-sensitive state of Ca V2 Ca 2+ channels, J Biol Chem, 279, 14619 (2004).
15. Rzepecki, P. W., and Prestwich, G. D., Synthesis of hybrid lipid probes: derivatives of phosphatidylethanolamine-extended phosphatidylinositol 4,5-bisphosphate (Pea-PIP(2)), J Org Chem, 67, 5454 (2002).
16. Schubert, M., Gautam, D., Surjo, D., Ueki, K., Baudler, S., Schubert, D., Kondo, T., Alber, J., Galldiks, N., Kustermann, E., Arndt, S., Jacobs, A. H., Krone, W., Kahn, C. R., and Bruning, J. C., Role for neuronal insulin resistance in neurodegenerative diseases, Proc Natl Acad Sci U S A, 101, 3100 (2004).
17. Srinivasan, S., Wang, F., Glavas, S., Ott, A., Hofmann, F., Aktories, K., Kalman, D., and Bourne, H. R., Rac and Cdc42 play distinct roles in regulating PI(3,4,5)P3 and polarity during neutrophil chemotaxis, J Cell Biol, 160, 375 (2003).
18. Sweeney, G., Garg, R. R., Ceddia, R. B., Li, D., Ishiki, M., Somwar, R., Foster, L. J., Neilsen, P. O., Prestwich, G. D., Rudich, A., and Klip, A., Intracellular delivery of phosphatidylinositol (3,4,5)-trisphosphate causes incorporation of glucose transporter 4 into the plasma membrane of muscle and fat cells without increasing glucose uptake, J Biol Chem, 279, 32233 (2004).
19. Terebiznik, M. R., Vieira, O. V., Marcus, S. L., Slade, A., Yip, C. M., Trimble, W. S., Meyer, T., Finlay, B. B., and Grinstein, S., Elimination of host cell PtdIns(4,5)P(2) by bacterial SigD promotes membrane fission during invasion by Salmonella, Nat Cell Biol, 4, 766 (2002).
20. Thomas, C. L., Steele, J., Prestwich, G. D., and Schiavo, G., Generation of phosphatidylinositol-specific antibodies and their characterization, Biochemical Society Transactions, 27, 648 (1999).
21. Wang, F., Herzmark, P., Weiner, O. D., Srinivasan, S., Servant, G., and Bourne, H. R., Lipid products of PI(3)Ks maintain persistent cell polarity and directed motility in neutrophils, Nat Cell Biol, 4, 513 (2002).
22. Ward, D. M., Shiflett, S. L., Huynh, D., Vaughn, M. B., Prestwich, G., and Kaplan, J., Use of Expression Constructs to Dissect the Functional Domains of the CHS/Beige Protein: Identification of Multiple Phenotypes, Traffic, 4, 403 (2003).
23. Weiner, O. D., Neilsen, P. O., Prestwich, G. D., Kirschner, M. W., Cantley, L. C., and Bourne, H. R., A PtdInsP(3)- and Rho GTPase-mediated positive feedback loop regulates neutrophil polarity, Nat Cell Biol, 4, 509 (2002).

PIP Beads

1. Casamayor, A., and Snyder, M., Molecular dissection of a yeast septin: distinct domains are required for septin interaction, localization, and function, Mol Cell Biol, 23, 2762 (2003).
2. Gupta, S., Fanzo, J. C., Hu, C., Cox, D., Jang, S. Y., Lee, A. E., Greenberg, S., and Pernis, A. B., T cell receptor engagement leads to the recruitment of IBP, a novel guanine nucleotide exchange factor, to the immunological synapse, J Biol Chem, 278, 43541 (2003).
3. Hiromura, M., Okada, F., Obata, T., Auguin, D., Shibata, T., Roumenstand, C., and Noguchi, M., Inhibition of Akt kinase activity by a peptide spanning the beta A strand of the protooncogene TCL1, J. Biol. Chem., M403775200 (2004).
4. Lee, S. J., Xu, H., Kang, L. W., Amzel, L. M., and Montell, C., Light adaptation through phosphoinositide-regulated translocation of Drosophila visual arrestin, Neuron, 39, 121 (2003).
5. Park, H. S., Lee, S. H., Park, D., Lee, J. S., Ryu, S. H., Lee, W. J., Rhee, S. G., and Bae, Y. S., Sequential activation of phosphatidylinositol 3-kinase, beta Pix, Rac1, and Nox1 in growth factor-induced production of H2O2, Mol Cell Biol, 24, 4384 (2004).
6. Wang, Y. J., Wang, J., Sun, H. Q., Martinez, M., Sun, Y. X., Macia, E., Kirchhausen, T., Albanesi, J. P., Roth, M. G., and Yin, H. L., Phosphatidylinositol 4 phosphate regulates targeting of clathrin adaptor AP-1 complexes to the Golgi, Cell, 114, 299 (2003).

PIP Strips

1. Abram, C. L., Seals, D. F., Pass, I., Salinsky, D., Maurer, L., Roth, T. M., and Courtneidge, S. A., The Adaptor Protein Fish Associates with Members of the ADAMs Family and Localizes to Podosomes of Src-transformed Cells, J Biol Chem, 278, 16844 (2003).
2. Aguilar, R. C., Watson, H. A., and Wendland, B., The yeast Epsin Ent1 is recruited to membranes through multiple independent interactions, J Biol Chem, 278, 10737 (2003).
3. Bompard, G., Martin, M., Roy, C., Vignon, F., and Freiss, G., Membrane targeting of protein tyrosine phosphatase PTPL1 through its FERM domain via binding to phosphatidylinositol 4,5-biphosphate, J Cell Sci, 116, 2519 (2003).
4. Burda, P., Padilla, S. M., Sarkar, S., and Emr, S. D., Retromer function in endosome-to-Golgi retrograde transport is regulated by the yeast Vps34 PtdIns 3-kinase, J Cell Sci, 115, 3889 (2002).
5. Casamayor, A., and Snyder, M., Molecular dissection of a yeast septin: distinct domains are required for septin interaction, localization, and function, Mol Cell Biol, 23, 2762 (2003).
6. Chang, J. S., Kim, S. K., Kwon, T. K., Bae, S. S., Min do, S., Lee, Y. H., Kim, S. O., Seo, J. K., Choi, J. H., and Suh, P. G., Pleckstrin Homology Domains of Phospholipase C-{gamma}1 Directly Interact with {beta}-Tubulin for Activation of Phospholipase C-{gamma}1 and Reciprocal Modulation of {beta}-Tubulin Function in Microtubule Assembly, J Biol Chem, 280, 6897 (2005).
7. Du, G., Altshuller, Y. M., Vitale, N., Huang, P., Chasserot-Golaz, S., Morris, A. J., Bader, M. F., and Frohman, M. A., Regulation of phospholipase D1 subcellular cycling through coordination of multiple membrane association motifs, J Cell Biol, 162, 305 (2003).
8. Dunn, R., Klos, D. A., Adler, A. S., and Hicke, L., The C2 domain of the Rsp5 ubiquitin ligase binds membrane phosphoinositides and directs ubiquitination of endosomal cargo, J Cell Biol, 165, 135 (2004).
9. Friant, S., Pecheur, E. I., Eugster, A., Michel, F., Lefkir, Y., Nourrisson, D., and Letourneur, F., Ent3p Is a PtdIns(3,5)P2 effector required for protein sorting to the multivesicular body, Dev Cell, 5, 499 (2003).
10. Fuentes, E. J., Karnoub, A. E., Booden, M. A., Der, C. J., and Campbell, S. L., Critical role of the pleckstrin homology domain in Dbs signaling and growth regulation, J Biol Chem, 278, 21188 (2003).
11. Gozani, O., Karuman, P., Jones, D. R., Ivanov, D., Cha, J., Lugovskoy, A. A., Baird, C. L., Zhu, H., Field, S. J., Lessnick, S. L., Villasenor, J., Mehrotra, B., Chen, J., Rao, V. R., Brugge, J. S., Ferguson, C. G., Payrastre, B., Myszka, D. G., Cantley, L. C., Wagner, G., Divecha, N., Prestwich, G. D., and Yuan, J., The PHD finger of the chromatin-associated protein ING2 functions as a nuclear phosphoinositide receptor, Cell, 114, 99 (2003).
12. Grishanin, R. N., Klenchin, V. A., Loyet, K. M., Kowalchyk, J. A., Ann, K., and Martin, T. F., Membrane association domains in Ca2+-dependent activator protein for secretion mediate plasma membrane and dense-core vesicle binding required for Ca2+-dependent exocytosis, J Biol Chem, 277, 22025 (2002).
13. Hanada, K., Kumagai, K., Yasuda, S., Miura, Y., Kawano, M., Fukasawa, M., and Nishijima, M., Molecular machinery for non-vesicular trafficking of ceramide, Nature, 426, 803 (2003).
14. Hanson, B. J., and Hong, W., Evidence for a role of SNX16 in regulating traffic between the early and later endosomal compartments, J Biol Chem, 278, 34617 (2003).
15. Hayashi, S., Okada, T., Igarashi, N., Fujita, T., Jahangeer, S., and Nakamura, S., Identification and characterization of RPK118, a novel sphingosine kinase-1-binding protein, J Biol Chem, 277, 33319 (2002).
16. Hayes, M. J., Merrifield, C. J., Shao, D., Ayala-Sanmartin, J., Schorey, C. D., Levine, T. P., Proust, J., Curran, J., Bailly, M., and Moss, S. E., Annexin 2 binding to phosphatidylinositol 4,5-bisphosphate on endocytic vesicles is regulated by the stress response pathway, J Biol Chem, 279, 14157 (2004).
17. Hilpela, P., Oberbanscheidt, P., Hahne, P., Hund, M., Kalhammer, G., Small, J. V., and Bahler, M., SWAP-70 identifies a transitional subset of actin filaments in motile cells, Mol Biol Cell, 14, 3242 (2003).
18. Hyun, T. S., Rao, D. S., Saint-Dic, D., Michael, L. E., Kumar, P. D., Bradley, S. V., Mizukami, I. F., Oravecz-Wilson, K. I., and Ross, T. S., HIP1 and HIP1r stabilize receptor tyrosine kinases and bind 3-phosphoinositides via epsin N-terminal homology domains, J Biol Chem, 279, 14294 (2004).
19. Ishii, M., Inanobe, A., and Kurachi, Y., PIP3 inhibition of RGS protein and its reversal by Ca2+/calmodulin mediate voltage-dependent control of the G protein cycle in a cardiac K+ channel, Proc Natl Acad Sci U S A, 99, 4325 (2002).
20. Johnson, L. M., James, K. M., Chamberlain, M. D., and Anderson, D. H., Identification of Key Residues in the A-Raf Kinase Important for Phosphoinositide Lipid Binding Specificity, Biochemistry, 44, 3432 (2005).
21. Klockow, B., Tichelaar, W., Madden, D. R., Niemann, H. H., Akiba, T., Hirose, K., and Manstein, D. J., The dynamin A ring complex: molecular organization and nucleotide-dependent conformational changes, Embo J, 21, 240 (2002).
22. Kostenko, E. V., Mahon, G. M., Cheng, L., and Whitehead, I. P., The Sec14 Homology Domain Regulates the Cellular Distribution and Transforming Activity of the Rho-specific Guanine Nucleotide Exchange Factor Dbs, J Biol Chem, 280, 2807 (2005).
23. Kruljac-Letunic, A., Moelleken, J., Kallin, A., Wieland, F., and Blaukat, A., The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1, J Biol Chem, 278, 29560 (2003).
24. Krylova, I. N., Sablin, E. P., Moore, J., Xu, R. X., Waitt, G. M., MacKay, J. A., Juzumiene, D., Bynum, J. M., Madauss, K., Montana, V., Lebedeva, L., Suzawa, M., Williams, J. D., Williams, S. P., Guy, R. K., Thornton, J. W., Fletterick, R. J., Willson, T. M., and Ingraham, H. A., Structural analyses reveal phosphatidyl inositols as ligands for the NR5 orphan receptors SF-1 and LRH-1, Cell, 120, 343 (2005).
25. Lee, S. J., Xu, H., Kang, L. W., Amzel, L. M., and Montell, C., Light adaptation through phosphoinositide-regulated translocation of Drosophila visual arrestin, Neuron, 39, 121 (2003).
26. Lee, S. B., Varnai, P., Balla, A., Jalink, K., Rhee, S. G., and Balla, T., The pleckstrin homology domain of phosphoinositide-specific phospholipase Cdelta4 is not a critical determinant of the membrane localization of the enzyme, J Biol Chem, 279, 24362 (2004).
27. Lee, S. J., Xu, H., and Montell, C., Rhodopsin kinase activity modulates the amplitude of the visual response in Drosophila, Proc Natl Acad Sci U S A, 101, 11874 (2004).
28. Lim, K. P., and Hong, W., Human Nischarin/Imidazoline Receptor Antisera-selected Protein Is Targeted to the Endosomes by a Combined Action of a PX Domain and a Coiled-coil Region, J Biol Chem, 279, 54770 (2004).
29. Lin, Y., Kimpler, L. A., Naismith, T. V., Lauer, J. M., and Hanson, P. I., Interaction of the Mammalian Endosomal Sorting Complex Required for Transport (ESCRT) III Protein hSnf7-1 with Itself, Membranes, and the AAA+ ATPase SKD1, J Biol Chem, 280, 12799 (2005).
30. McDonald, E. R., 3rd, and El-Deiry, W. S., Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth, Proc Natl Acad Sci U S A, 101, 6170 (2004).
31. Mills, I. G., Praefcke, G. J., Vallis, Y., Peter, B. J., Olesen, L. E., Gallop, J. L., Butler, P. J., Evans, P. R., and McMahon, H. T., EpsinR: an AP1/clathrin interacting protein involved in vesicle trafficking, J Cell Biol, 160, 213 (2003).
32. Olsten, M. E. K., Canton, D. A., Zhang, C., Walton, P. A., and Litchfield, D. W., The Pleckstrin Homology Domain of CK2 Interacting Protein-1 Is Required for Interactions and Recruitment of Protein Kinase CK2 to the Plasma Membrane, J. Biol. Chem., 279, 42114 (2004).
33. Rohde, H. M., Cheong, F. Y., Konrad, G., Paiha, K., Mayinger, P., and Boehmelt, G., The human phosphatidylinositol phosphatase SAC1 interacts with the coatomer I complex, J Biol Chem, 278, 52689 (2003).
34. Safi, A., Vandromme, M., Caussanel, S., Valdacci, L., Baas, D., Vidal, M., Brun, G., Schaeffer, L., and Goillot, E., Role for the pleckstrin homology domain-containing protein CKIP-1 in phosphatidylinositol 3-kinase-regulated muscle differentiation, Mol Cell Biol, 24, 1245 (2004).
35. Santagata, S., Boggon, T. J., Baird, C. L., Gomez, C. A., Zhao, J., Shan, W. S., Myszka, D. G., and Shapiro, L., G-protein signaling through tubby proteins, Science, 292, 2041 (2001).
36. Sato, M., Ueda, Y., Takagi, T., and Umezawa, Y., Production of PtdInsP3 at endomembranes is triggered by receptor endocytosis, Nat Cell Biol, 5, 1016 (2003).
37. Saxena, A., Morozov, P., Frank, D., Musalo, R., Lemmon, M. A., Skolnik, E. Y., and Tycko, B., Phosphoinositide binding by the pleckstrin homology domains of Ipl and Tih1, J Biol Chem, 277, 49935 (2002).
38. Schulz, T. A., and Creutz, C. E., The tricalbin C2 domains: lipid-binding properties of a novel, synaptotagmin-like yeast protein family, Biochemistry, 43, 3987 (2004).39. Shen, T. L., Han, D. C., and Guan, J. L., Association of Grb7 with phosphoinositides and its role in the regulation of cell migration, J Biol Chem, 277, 29069 (2002).
39. Shen, T. L., Han, D. C., and Guan, J. L., Association of Grb7 with phosphoinositides and its role in the regulation of cell migration, J Biol Chem, 277, 29069 (2002).
40. Simonsen, A., Birkeland, H. C. G., Gillooly, D. J., Mizushima, N., Kuma, A., Yoshimori, T., Slagsvold, T., Brech, A., and Stenmark, H., Alfy, a novel FYVE-domain-containing protein associated with protein granules and autophagic membranes, J. Cell Sci., 117, 4239 (2004).
41. Six, D. A., and Dennis, E. A., Essential Ca2+-independent role of the group IVA cytosolic phospholipase A2 C2 domain for interfacial activity, J Biol Chem, 278, 23842 (2003).
42. Skowronek, K. R., Guo, F., Zheng, Y., and Nassar, N., The C-terminal basic tail of RhoG assists the guanine nucleotide exchange factor Trio in binding to phospholipids, J Biol Chem (2004).
43. Stromhaug, P. E., Reggiori, F., Guan, J., Wang, C.-W., and Klionsky, D. J., Atg21 Is a Phosphoinositide Binding Protein Required for Efficient Lipidation and Localization of Atg8 during Uptake of Aminopeptidase I by Selective Autophagy, Mol. Biol. Cell, 15, 3553 (2004).
44. Takano, N., Owada, Y., Suzuki, R., Sakagami, H., Shimosegawa, T., and Kondo, H., Cloning and characterization of a novel variant (mM-rdgBbeta1) of mouse M-rdgBs, mammalian homologs of Drosophila retinal degeneration B gene proteins, and its mRNA localization in mouse brain in comparison with other M-rdgBs, J Neurochem, 84, 829 (2003).
45. Thapar, R., Karnoub, A. E., and Campbell, S. L., Structural and biophysical insights into the role of the insert region in Rac1 function, Biochemistry, 41, 3875 (2002).
46. Varnai, P., Lin, X., Lee, S. B., Tuymetova, G., Bondeva, T., Spat, A., Rhee, S. G., Hajnoczky, G., and Balla, T., Inositol lipid binding and membrane localization of isolated pleckstrin homology (PH) domains. Studies on the PH domains of phospholipase C delta 1 and p130, J Biol Chem, 277, 27412 (2002).
47. Virbasius, J. V., Song, X., Pomerleau, D. P., Zhan, Y., Zhou, G. W., and Czech, M. P., Activation of the Akt-related cytokine-independent survival kinase requires interaction of its phox domain with endosomal phosphatidylinositol 3-phosphate, Proc Natl Acad Sci U S A, 98, 12908 (2001).
48. Wild, A. C., Yu, J. W., Lemmon, M. A., and Blumer, K. J., The p21-activated Protein Kinase-related Kinase Cla4 Is a Coincidence Detector of Signaling by Cdc42 and Phosphatidylinositol 4-Phosphate, J Biol Chem, 279, 17101 (2004).
49. Xu, J., Liu, D., Gill, G., and Songyang, Z., Regulation of cytokine-independent survival kinase (CISK) by the Phox homology domain and phosphoinositides, J Cell Biol, 154, 699 (2001).
50. Zhong, Q., Lazar, C. S., Tronchere, H., Sato, T., Meerloo, T., Yeo, M., Songyang, Z., Emr, S. D., and Gill, G. N., Endosomal localization and function of sorting nexin 1, Proc Natl Acad Sci U S A, 99, 6767 (2002).

PIP Arrays

1. Abram, C. L., Seals, D. F., Pass, I., Salinsky, D., Maurer, L., Roth, T. M., and Courtneidge, S. A., The Adaptor Protein Fish Associates with Members of the ADAMs Family and Localizes to Podosomes of Src-transformed Cells, J Biol Chem, 278, 16844 (2003).
2. Berger, P., Schaffitzel, C., Berger, I., Ban, N., and Suter, U., Membrane association of myotubularin-related protein 2 is mediated by a pleckstrin homology-GRAM domain and a coiled-coil dimerization module, Proc Natl Acad Sci U S A, 100, 12177 (2003).
3. Cheever, M. L., Sato, T. K., de Beer, T., Kutateladze, T. G., Emr, S. D., and Overduin, M., Phox domain interaction with PtdIns(3)P targets the Vam7 t-SNARE to vacuole membranes, Nat Cell Biol, 3, 613 (2001).
4. Cheng, G., and Lambeth, J. D., NOXO1, regulation of lipid binding, localization, and activation of Nox1 by the Phox homology (PX) domain, J Biol Chem, 279, 4737 (2004).
5. Gozani, O., Karuman, P., Jones, D. R., Ivanov, D., Cha, J., Lugovskoy, A. A., Baird, C. L., Zhu, H., Field, S. J., Lessnick, S. L., Villasenor, J., Mehrotra, B., Chen, J., Rao, V. R., Brugge, J. S., Ferguson, C. G., Payrastre, B., Myszka, D. G., Cantley, L. C., Wagner, G., Divecha, N., Prestwich, G. D., and Yuan, J., The PHD finger of the chromatin-associated protein ING2 functions as a nuclear phosphoinositide receptor, Cell, 114, 99 (2003).
6. Hirst, J., Motley, A., Harasaki, K., Peak Chew, S. Y., and Robinson, M. S., EpsinR: an ENTH domain-containing protein that interacts with AP-1, Mol Biol Cell, 14, 625 (2003).
7. Lindsay, A. J., and McCaffrey, M. W., The C2 domains of the class I Rab11 family of interacting proteins target recycling vesicles to the plasma membrane, J. Cell Sci., 117, 4365 (2004).
8. Mishra, S. K., Keyel, P. A., Hawryluk, M. J., Agostinelli, N. R., Watkins, S. C., and Traub, L. M., Disabled-2 exhibits the properties of a cargo-selective endocytic clathrin adaptor, Embo J, 21, 4915 (2002).
9. Santagata, S., Boggon, T. J., Baird, C. L., Gomez, C. A., Zhao, J., Shan, W. S., Myszka, D. G., and Shapiro, L., G-protein signaling through tubby proteins, Science, 292, 2041 (2001).
10. Saxena, A., Morozov, P., Frank, D., Musalo, R., Lemmon, M. A., Skolnik, E. Y., and Tycko, B., Phosphoinositide binding by the pleckstrin homology domains of Ipl and Tih1, J Biol Chem, 277, 49935 (2002).
11. Tsujita, K., Itoh, T., Ijuin, T., Yamamoto, A., Shisheva, A., Laporte, J., and Takenawa, T., Myotubularin regulates the function of the late endosome through the gram domain-phosphatidylinositol 3,5-bisphosphate interaction, J Biol Chem, 279, 13817 (2004).
12. Wang, Q., Xie, Y., Du, Q. S., Wu, X. J., Feng, X., Mei, L., McDonald, J. M., and Xiong, W. C., Regulation of the formation of osteoclastic actin rings by proline-rich tyrosine kinase 2 interacting with gelsolin, J Cell Biol, 160, 565 (2003).
13. Xu, J., Liu, D., Gill, G., and Songyang, Z., Regulation of cytokine-independent survival kinase (CISK) by the Phox homology domain and phosphoinositides, J Cell Biol, 154, 699 (2001).
14. Zhan, Y., Virbasius, J. V., Song, X., Pomerleau, D. P., and Zhou, G. W., The p40phox and p47phox PX domains of NADPH oxidase target cell membranes via direct and indirect recruitment by phosphoinositides, J Biol Chem, 277, 4512 (2002).

Shuttle PIP

1. Chen, G., Raman, P., Bhonagiri, P., Strawbridge, A. B., Pattar, G. R., and Elmendorf, J. S., Protective Effect of Phosphatidylinositol 4,5-Bisphosphate against Cortical Filamentous Actin Loss and Insulin Resistance Induced by Sustained Exposure of 3T3-L1 Adipocytes to Insulin, J. Biol. Chem., 279, 39705 (2004).
2. Hayes, M. J., Merrifield, C. J., Shao, D., Ayala-Sanmartin, J., Schorey, C. D., Levine, T. P., Proust, J., Curran, J., Bailly, M., and Moss, S. E., Annexin 2 binding to phosphatidylinositol 4,5-bisphosphate on endocytic vesicles is regulated by the stress response pathway, J Biol Chem, 279, 14157 (2004).
3. Larsen, M., Hoffman, M. P., Sakai, T., Neibaur, J. C., Mitchell, J. M., and Yamada, K. M., Role of PI 3-kinase and PIP3 in submandibular gland branching morphogenesis, Dev Biol, 255, 178 (2003).
4. Maffucci, T., Brancaccio, A., Piccolo, E., Stein, R. C., and Falasca, M., Insulin induces phosphatidylinositol-3-phosphate formation through TC10 activation, Embo J, 22, 4178 (2003).
5. Markadieu, N., Blero, D., Boom, A., Erneux, C., and Beauwens, R., Phosphatidylinositol 3,4,5-trisphosphate: an early mediator of insulin-stimulated sodium transport in A6 cells, Am J Physiol Renal Physiol, 287, F319 (2004).
6. Nigorikawa, K., Okamura, N., and Hazeki, O., The effect of anionic amphiphiles on the recruitment of rac in neutrophils, J Biochem (Tokyo), 136, 463 (2004).
7. Ortiz, P. A., Hong, N. J., and Garvin, J. L., Luminal flow induces eNOS activation and translocation in the rat thick ascending limb. II. Role of PI3-kinase and Hsp90, Am J Physiol Renal Physiol, 287, F281 (2004).
8. Ozaki, S., DeWald, D. B., Shope, J. C., Chen, J., and Prestwich, G. D., Intracellular delivery of phosphoinositides and inositol phosphates using polyamine carriers, Proc Natl Acad Sci U S A, 97, 11286 (2000).
9. Soughayer, J. S., Wang, Y., Li, H., Cheung, S. H., Rossi, F. M., Stanbridge, E. J., Sims, C. E., and Allbritton, N. L., Characterization of TAT-mediated transport of detachable kinase substrates, Biochemistry, 43, 8528 (2004).
10. Stahelin, R. V., Burian, A., Bruzik, K. S., Murray, D., and Cho, W., Membrane binding mechanisms of the PX domains of NADPH oxidase p40phox and p47phox, J Biol Chem, 278, 14469 (2003).
11. Stratford, S., Hoehn, K. L., Liu, F., and Summers, S. A., Regulation of Insulin Action by Ceramide: DUAL MECHANISMS LINKING CERAMIDE ACCUMULATION TO THE INHIBITION OF Akt/PROTEIN KINASE B, J Biol Chem, 279, 36608 (2004).
12. Sweeney, G., Garg, R. R., Ceddia, R. B., Li, D., Ishiki, M., Somwar, R., Foster, L. J., Neilsen, P. O., Prestwich, G. D., Rudich, A., and Klip, A., Intracellular delivery of phosphatidylinositol (3,4,5)-trisphosphate causes incorporation of glucose transporter 4 into the plasma membrane of muscle and fat cells without increasing glucose uptake, J Biol Chem, 279, 32233 (2004).
13. Tong, Q., Gamper, N., Medina, J. L., Shapiro, M. S., and Stockand, J. D., Direct activation of the epithelial Na(+) channel by phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate produced by phosphoinositide 3-OH kinase, J Biol Chem, 279, 22654 (2004).
14. Wang, Y. J., Wang, J., Sun, H. Q., Martinez, M., Sun, Y. X., Macia, E., Kirchhausen, T., Albanesi, J. P., Roth, M. G., and Yin, H. L., Phosphatidylinositol 4 phosphate regulates targeting of clathrin adaptor AP-1 complexes to the Golgi, Cell, 114, 299 (2003).
15. Wang, Y. J., Li, W. H., Wang, J., Xu, K., Dong, P., Luo, X., and Yin, H. L., Critical role of PIP5KI{gamma}87 in InsP3-mediated Ca(2+) signaling, J Cell Biol, 167, 1005 (2004).
16. Weiner, O. D., Neilsen, P. O., Prestwich, G. D., Kirschner, M. W., Cantley, L. C., and Bourne, H. R., A PtdInsP(3)- and Rho GTPase-mediated positive feedback loop regulates neutrophil polarity, Nat Cell Biol, 4, 509 (2002).

PI3-Kinase ELISA

1. CAO, X., KAMBE, F., MOELLER, L. C., REFETOFF, S., and SEO, H., Thyroid hormone induces rapid activation of Akt/PKB-mTOR-p70S6K cascade through PI3K in human fibroblasts, Mol. Endocrinol., me.2004 (2004).
2. Flaxenburg, J. A., Melter, M., Lapchak, P. H., Briscoe, D. M., and Pal, S., The CD40-induced signaling pathway in endothelial cells resulting in the overexpression of vascular endothelial growth factor involves Ras and phosphatidylinositol 3-kinase, J Immunol, 172, 7503 (2004).
3. Lee, T. K., Man, K., Ho, J. W., Sun, C. K., Ng, K. T., Wang, X. H., Wong, Y. C., Ng, I. O., Xu, R. R., and Fan, S. T., FTY720 induces apoptosis of human hepatoma cell lines through PI3-K-mediated Akt dephosphorylation, Carcinogenesis, bgh250 (2004).
4. Oganesian, A., Poot, M., Daum, G., Coats, S. A., Wright, M. B., Seifert, R. A., and Bowen-Pope, D. F., Protein tyrosine phosphatase RQ is a phosphatidylinositol phosphatase that can regulate cell survival and proliferation, Proc Natl Acad Sci U S A, 100, 7563 (2003).
5. Shi, H., Tzameli, I., Bjorbaek, C., and Flier, J. S., Suppressor of cytokine signaling 3 is a physiological regulator of adipocyte insulin signaling, J Biol Chem, 279, 34733 (2004).

Isoprenoids

1. Adam, P., Hecht, S., Eisenreich, W., Kaiser, J., Grawert, T., Arigoni, D., Bacher, A., and Rohdich, F., Biosynthesis of terpenes: studies on 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase, Proc Natl Acad Sci U S A, 99, 12108 (2002).
2. Bailey, A. M., Mahapatra, S., Brennan, P. J., and Crick, D. C., Identification, cloning, purification, and enzymatic characterization of Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate synthase, Glycobiology, 12, 813 (2002).
3. Burke, C., and Croteau, R., Interaction with the small subunit of geranyl diphosphate synthase modifies the chain length specificity of geranylgeranyl diphosphate synthase to produce geranyl diphosphate, J Biol Chem, 277, 3141 (2002).
4. Chen, F., Tholl, D., D'Auria, J. C., Farooq, A., Pichersky, E., and Gershenzon, J., Biosynthesis and emission of terpenoid volatiles from Arabidopsis flowers, Plant Cell, 15, 481 (2003).
5. Dudareva, N., Martin, D., Kish, C. M., Kolosova, N., Gorenstein, N., Faldt, J., Miller, B., and Bohlmann, J., (E)-beta-ocimene and myrcene synthase genes of floral scent biosynthesis in snapdragon: function and expression of three terpene synthase genes of a new terpene synthase subfamily, Plant Cell, 15, 1227 (2003).
6. Gottlin, E. B., Benson, R. E., Conary, S., Antonio, B., Duke, K., Payne, E. S., Ashraf, S. S., and Christensen, D. J., High-throughput screen for inhibitors of 1-deoxy-d-xylulose 5-phosphate reductoisomerase by surrogate ligand competition, J Biomol Screen, 8, 332 (2003).
7. Iijima, Y., Gang, D. R., Fridman, E., Lewinsohn, E., and Pichersky, E., Characterization of geraniol synthase from the peltate glands of sweet basil, Plant Physiol, 134, 370 (2004).
8. Martin, D., Tholl, D., Gershenzon, J., and Bohlmann, J., Methyl jasmonate induces traumatic resin ducts, terpenoid resin biosynthesis, and terpenoid accumulation in developing xylem of Norway spruce stems, Plant Physiol, 129, 1003 (2002).
9. Martin, D. M., Gershenzon, J., and Bohlmann, J., Induction of volatile terpene biosynthesis and diurnal emission by methyl jasmonate in foliage of Norway spruce, Plant Physiol, 132, 1586 (2003).
10. McKay, S. A., Hunter, W. L., Godard, K. A., Wang, S. X., Martin, D. M., Bohlmann, J., and Plant, A. L., Insect attack and wounding induce traumatic resin duct development and gene expression of (-)-pinene synthase in Sitka spruce, Plant Physiol, 133, 368 (2003).
11. Rohdich, F., Hecht, S., Gartner, K., Adam, P., Krieger, C., Amslinger, S., Arigoni, D., Bacher, A., and Eisenreich, W., Studies on the nonmevalonate terpene biosynthetic pathway: metabolic role of IspH (LytB) protein, Proc Natl Acad Sci U S A, 99, 1158 (2002).
12. Tholl, D., Kish, C. M., Orlova, I., Sherman, D., Gershenzon, J., Pichersky, E., and Dudareva, N., Formation of monoterpenes in Antirrhinum majus and Clarkia breweri flowers involves heterodimeric geranyl diphosphate synthases, Plant Cell, 16, 977 (2004).
13. Voynova, N. E., Rios, S. E., and Miziorko, H. M., Staphylococcus aureus mevalonate kinase: isolation and characterization of an enzyme of the isoprenoid biosynthetic pathway, J Bacteriol, 186, 61 (2004).
14. Wouters, J., Oudjama, Y., Ghosh, S., Stalon, V., Droogmans, L., and Oldfield, E., Structure and mechanism of action of isopentenylpyrophosphate- dimethylallylpyrophosphate isomerase, J Am Chem Soc, 125, 3198 (2003).
15. Buurman, E. T., Blodgett, A. E., Hull, K. G., and Carcanague, D., Pyridines and pyrimidines mediating activity against an efflux-negative strain of Candida albicans through putative inhibition of lanosterol demethylase, Antimicrob Agents Chemother, 48, 313 (2004).
16. Holstein, S. A., Wohlford-Lenane, C. L., Wiemer, D. F., and Hohl, R. J., Isoprenoid pyrophosphate analogues regulate expression of Ras-related proteins, Biochemistry, 42, 4384 (2003).
17. Shinoda, T., and Itoyama, K., Juvenile hormone acid methyltransferase: a key regulatory enzyme for insect metamorphosis, Proc Natl Acad Sci U S A, 100, 11986 (2003).

Isoprenoids

1. Bagga, S., Price, K. S., Lin, D. A., Friend, D. S., Austen, K. F., and Boyce, J. A., Lysophosphatidic acid accelerates the development of human mast cells, Blood, 2004 (2004).