Echelon's High Throughput Autotaxin Inhibitor Assay uses purified autotaxin and the autotaxin substrate FS-3, which fluoresces upon cleavage.  The assay is performed in a 384 well plate and quantifies the autotaxin activity in the presence of the user's samples by measuring changes in fluorescence over time.  Percent autotaxin inhibition is determined by referencing no inhibitor controls.  The autotaxin inhibitor, BrP-LPA, is supplied to positively control for autotaxin inhibition.  The assay is resistant to modest concentrations of DMSO and is performed in a continuous assay format, which has the advantage of being less susceptible to error arrising from mis-timing the addition of components to different sample wells.