**Service for this assay has been discontinued and is no longer available
The Sphingomyelin Assay Service provides a sensitive and homogenous method for the quantification of sphingomyelin. In this enzyme coupled assay sphingomyelin is cleaved into phosphorylcholine and n-acylsphingosine using Sphingomyelinase. Alkaline Phosphatase hydrolyzes phosphorylcholine and choline is oxidized by choline oxidase to hydrogen peroxide. DAOS plus 4-Aminoantipyrine (4 AAP) in the presence of hydrogen peroxide and peroxidase results in the oxidative coupling of DAOS and 4 AAP to form a blue chromogen that is measured by absorbance at 595 nm1. Experiments have shown the Sphingomyelin Assay Service detects sphingomyelin at concentrations from 17 to 100 mg/mL.
Please refer to the technical data sheet for sample considerations and shipping information. To place an order please contact Echelon at 801.588.0455.
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