**This product has been discontinued and is no longer available**

HDAC Assay Developer is an essential reagent for signal generation in HDAC or Sirtuin assays using a fluorogenic acetylated peptide substrate for HDACs based on the residues of 379-382 of p53 (Arg-His-Lys-Lys (Ac)), as site of regulatory acetylation by the p300 and CBP acetyltransferase (lysines 381, 382). An appropriate fluorogenic substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC Assay Developer produces a fluorophore. Typically, an ¿inhibitor stop¿ will be included in the HDAC Assay Developer (e.g. Trichostatin A for class I and II HDACs, nicotinamide for sirtuins).