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       Category : Assays :   PI3-Kinase Assay : Phosphatidylcholine Assay Kit
     Category : Assays :   Sphingomyelin/Phosphatidylcholine Assays : Phosphatidylcholine Assay Kit



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—A sensitive and homogenous method for the quantification of phosphatidylcholine

Description:
—In this enzyme coupled assay, phosphatidylcholine is cleaved by Phospholipase D to produce choline and phosphatidic acid. Choline is oxidized by choline oxidase to betain producing hydrogen peroxide. DAOS plus 4-Aminoantipyrine (4 AAP) in the presence of hydrogen peroxide and peroxidase results in the oxidative coupling of DAOS and 4 AAP to form a blue chromogen that is measured by absorbance at 595 nm1.
—Experiments have shown the Phosphatidylcholine Assay Kit can detect phosphatidylcholine at concentrations of 17 ug/mL using a reaction time of 4 hours.
—The kit is designed to quantify PC of 40 samples ran in duplicate.
Storage:
—neg 20° C
Catalog:
Sphingomyelin / Phosphatidylcholine Assays
 
Additional Informaiton
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K-31PC