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Product Name
Sphingomyelin Assay Kit
Catalog Number
K-31SM
Unit Price
$250.00
Quantity
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—A sensitive and homogenous method for the quantification of Sphingomyelin |
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| Description: |
—In this enzyme coupled assay, sphingomyelin is cleaved into phosphorylcholine and n-acylsphingosine using Sphingomyelinase. Alkaline Phosphatase hydrolyzes phosphorylcholine and choline is oxidized by choline oxidase to hydrogen peroxide. DAOS plus 4-Aminoantipyrine (4 AAP) in the presence of hydrogen peroxide and peroxidase results in the oxidative coupling of DAOS and 4 AAP to form a blue chromogen that is measured by absorbance at 595 nm1.
—Experiments have shown the Sphingomyelin Assay Kit can detect sphingomyelin at concentrations of 17 ug/mL using a reaction time of 4 hours.
—The kit is designed to quantify SM of 40 samples ran in duplicate.
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| Storage: |
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—neg 20° C
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| Catalog: |
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—Sphingomyelin / Phosphatidylcholine Assays
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