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Product Name
Akt2, active
Catalog Number
E-K002G
Unit Price
$225.00
Quantity
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Recombinant full-length human Akt2 containing was expressed by baculovirus in Sf 9 insect cells usring a N-terminal GST tag.
The gene accession number is NM_001626.
This material is sold for research purposes only. |
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| Scientific Background: |
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—Akt2 or Protein Kinase B α (PKBα) is a serine/threonine kinase that belongs to the Akt family. Akt2 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). The PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt2 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) (2).
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| Storage: |
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—Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoid repeated thawing and freezing.
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| References: |
—1. Coffer PJ, Jin J, Woodgett JR. Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 (Pt 1): 1-13.
2. Anderson KE, Coadwell J, Hawkins PT. Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.
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