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Product Name
CSK, active
Catalog Number
E-K015
Unit Price
$275.00
Quantity
10.0 ug
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Recombinant full length human CSK containing N-terminal GST tag was expressed by baculovirus in Sf 9 insect cells.
The gene accession number is NM_004383.
This material is sold for research purposes only.
Specific Activity
425 nmol phosphate incorporated into Poly(Glu-Tyr) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
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| Scientific Background: |
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—CSK is a cytoplasmic tyrosine kinase that has been shown to downregulate the tyrosine kinase activity of the c-src oncoprotein through tyrosine phosphorylation of the c-src carboxy terminus (1). Cell transformation by src oncoprotein is caused by several oncogenic mechanisms, which interfere with the carboxy terminal phosphorylation. The CSK could therefore potentially function as an anti-oncogene. CSK is ubiquitously expressed in human tissues as 2 mRNA species of 2.6 and 3.4 kb, although in some tissues and cell lines, only the larger mRNA is detected (2). A yeast 2-hybrid system has been used to identify proteins associated with CSK. The Src homology-3 (SH3) domain of CSK associates with a proline-rich region of PEP, a protein-tyrosine phosphatase expressed in hemopoietic cells (2). This association is highly specific and it is speculated that PEP may be an effector and/or regulator of CSK in T cells and other hemopoietic cells. An in situ hybridization has been used to map the CSK gene to chromosome 15q23-q25 (3).
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| Storage: |
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—Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoid repeated thawing and freezing.
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| References : |
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—1. Partanen, J.; Armstrong, E.; Bergman, M.; Makela, T. P.; Hirvonen, H.; Huebner, K.; Alitalo, K.: Cyl encodes a putative cytoplasmic tyrosine kinase lacking the conserved tyrosine autophosphorylation site (Y416-src). Oncogene 6: 2013-2018, 1991. 2. Cloutier, J.-F.; Veillette, A. : Association of inhibitory tyrosine protein kinase p50(csk) with protein tyrosine phosphatase PEP in T cells and other hemopoietic cells. EMBO J. 15: 4909-4918, 1996. 3. Armstrong, E.; Cannizzaro, L.; Bergman, M.; Huebner, K.; Alitalo, K.: The c-src tyrosine kinase (CSK) gene, a potential antioncogene, localizes to human chromosome region 15q23-q25. Cytogenet. Cell Genet. 60: 119-120, 1992.
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