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Product Name
CHK1, active
Catalog Number
E-K012
Unit Price
$225.00
Quantity
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Recombinant full length human CHK1 containing N-terminal GST tag was expressed by baculovirus in Sf 9 insect cells.
The gene accession number is NM_001274.
This material is sold for research purposes only.
Specific Activity
369 nmol phosphate incorporated into CHKtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
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| Scientific Background: |
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—CHK1 is a 56 kd serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle (1). CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia (2). In vitro, CHK1 binds to and phosphorylates the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases (3). CHK1 phosphorylates Cdc25C on serine-216 which creates a binding site for 14-3-3 protein and inhibits function of the phosphatase. Thus, in response to DNA damage, CHK1 phosphorylates and inhibits Cdc25C, thereby preventing the activation of the Cdc2-cyclin B complex and mitotic entry.
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| Storage: |
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—Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoid repeated thawing and freezing.
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| References : |
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—1. Walworth N, Davey S, Beach D. Fission yeast CHK1 protein kinase links the rad checkpoint pathway to cdc2. Nature. 1993 May 27;363(6427):368-71. 2. Walworth NC, Bernards R. rad-dependent response of the CHK1-encoded protein kinase at the DNA damage checkpoint. Science. 1996 Jan 19;271(5247):353-6. 3. Sanchez Y, Wong C, Thoma RS, Richman R, Wu Z, Piwnica-Worms H, Elledge SJ. Conservation of the CHK1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science. 1997 Sep 5;277(5331):1497-501.
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