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Product Name
CAMK4, active
Catalog Number
E-K011
Unit Price
$225.00
Quantity
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Recombinant full-length human CAMK4 containing N-terminal GST tag was expressed by baculovirus in Sf 9 insect cells.
The gene accession number is NM_001744.
This material is sold for research purposes only.
Specific Activity
83 nmol phosphate incorporated into Autocamtide 2 per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
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| Scientific Background: |
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—CAMK4 belongs to the serine/threonine protein kinase family and Ca(2+)/calmodulin- dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution. Sikela et al. identified that brain CaM kinase IV (CAMK4) appeared to be restricted to brain in the rat; interestingly, it was not detected in the brain of the newborn, but became detectable within a few days after birth. They also raised the possibility that a defect in Camk4 may be responsible for 1 of the mutant phenotypes in the centromeric region near 2 mutations, which known to affect neurologic function and fertility (1). Wei et al. found Camk4-labeled neurons in the hippocampus, amygdala, anterior cingulate cortex, somatosensory cortex, and insular cortex (2). Kang et al. generated transgenic mice in which the expression of a dominant-negative form of Camk4 was restricted to the postnatal forebrain. In these transgenic mice, activity-induced Creb phosphorylation and Fos expression were significantly attenuated. The results indicated that neural activity-dependent CAMK4 signaling in the neuronal nucleus plays an important role in the consolidation/retention of hippocampus-dependent long-term memory (3). Wu et al. generated transgenic mice that selectively express in skeletal muscle a constitutively active form of CAMK4 and concluded that a calcium-regulated signaling pathway controls mitochondrial biogenesis in mammalian cells (4). Wei et al. studied pain and fear memory in Camk4-null mice and concluded that Camk4 is crucial in the trapping of Ca2(+)/calmodulin complexes in neuronal nuclei and in Creb phosphorylation and activation (5).
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| Storage: |
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—Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoid repeated thawing and freezing.
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| References : |
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—1. Sikela, J. M.; Law, M. L.; Kao, F.-T.; Hartz, J. A.; Wei, Q.; Hahn, W. E.: Chromosomal localization of the human gene for brain Ca(2+)/calmodulin-dependent protein kinase type IV. Genomics 4: 21-27, 1989. 2. Wei, F.; Qiu, C.S.; Liauw, J.; Robinson, D. A.; Ho, N.; Chatila, T.; Zhuo, M.: Calcium-calmodulin-dependent protein kinase IV is required for fear memory. Nature Neurosci. 5: 573-579, 2002. 3. Kang, H.; Sun, L. D.; Atkins, C. M.; Soderling, T. R.; Wilson, M. A.; Tonegawa, S.: An important role of neural activity-dependent CaMKIV signaling in the consolidation of long-term memory. Cell 106: 771-783, 2001. 4. Wu, H.; Kanatous, S. B.; Thurmond, F. A.; Gallardo, T.; Isotani, E.; Bassel-Duby, R.; Williams, R. S.: Regulation of mitochondrial biogenesis in skeletal muscle by CaMK. Science 296: 349-352, 2002. 5. Wei, F.; Qiu, C.S.; Liauw, J.; Robinson, D. A.; Ho, N.; Chatila, T.; Zhuo, M.: Calcium-calmodulin-dependent protein kinase IV is required for fear memory. Nature Neurosci. 5: 573-579, 2002.
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