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Product Name
CAMK1G, active
Catalog Number
E-K010
Unit Price
$225.00
Quantity
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Recombinant full-length CAMK1G, containing N-terminal GST tag was expressed by
baculovirus in Sf 9 insect cells. The gene accession number is NM_020439. This material is sold for research purposes only.
Specific Acitivity
768 nmol phospha
minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
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| Scientific Background: |
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—CLICK-III/CaMKIG is protein kinase (CaMK), an isoform of the CaMKI family with an extended C-terminal domain ending with CAAX motif (where AA is aliphatic acid). As expected from the similarity of its kinase domain with the other CaMKI isoforms, full activation of CLICK-III/CaMKIG requiredboth Ca(2+)/CaM and phosphorylation by CaMKK. Ca(2+)/cAMP-response element-binding protein (CREB) was a good substrate for CLICK-III/CaMKIG, at least in vitro. Interestingly enough, CLICK-III/CaMKIG transcripts were most abundant in neurons, with the highest levein limited nuclei such as the central nucleus of the amygdala (CeA) and the ventromedial hypothalamus. Consistent with the presence of the CAAX motif, CLICK-III/CaMKIG wasto be anchored to various membrane compartments, especially to Golgi and plasma membranes. Both point mutation in the CAAX motif and treatment with compactin, a HMG-CoA reductase inhibitor, disrupted such membrane localization, suggesting that membrane localization of CLICK-III/CaMKIG occurred in a prenylation-dependent way. These findings provide a nomechanism by which neuronal CaMK activity could be targeted to specific membrane compartments.
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| Storage: |
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—Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoid repeated thawing and freezing.
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| References: |
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—1. Takemoto Furuyashiki,T., Narumiya,S. and Bito,H. Molecular cloning and characterization of CLICK-III/CaMKIgamma, a novel membrane-anchored neuronal Ca2+/calmodulin-dependent proteikinase (CaMK). J. Biol. Chem. 278 (20), 18597-18605 (2003)
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