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Product Name
CAMK1d, active
Catalog Number
E-K009
Unit Price
$225.00
Quantity
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Recombinant full-length human CAMK1D containing N-terminal GST tag was expressed by baculovirus in Sf 9 insect cells.
The gene accession number is NM_153498.
This material is sold for research purposes only.
Specific Activity
554 nmol phosphate incorporated into Autocamtide 2 per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
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| Scientific Background: |
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—Calcium/calmodulin-dependent protein kinase ID (CAMK1D), or a novel Ca2+/calmodulin-dependent kinase I-like kinase (CKLiK), showed kinase activity and that the activity was enhanced by Ca(2+) and calmodulin. Using a novel antibody generated against the C-terminus of CKLiK, CKLiK was detected in CD34+-derived neutrophils and eosinophils, as well as in mature peripheral blood granulocytes. Activation of human granulocytes by N-formyl-methionyl-leucyl-phenylalanine (fMLP) and platelet-activating factor (PAF), but not the phorbol ester PMA (phorbol 12-myristate-13-acetate), resulted in induction of CKLiK activity, in parallel with a rise of intracellular [Ca2+]. Furthermore, fMLP-induced neutrophil migration on albumin-coated surfaces was perturbed, as well as beta2-integrin-mediated adhesion. These findings suggest a critical role for CKLiK in modulating chemoattractant-induced functional responses in human granulocytes (1). Also, CAMK1D exhibits Ca(2+)/CaM-dependent activity that is enhanced (approximately 30-fold) in vitro by phosphorylation of its Thr180 by CaM-K kinase (CaM-KK)alpha, consistent with detection of CAMK1D-activating activity in HeLa cells. Transiently expressed CAMK1D exhibited enhanced protein kinase activity in HeLa cells without ionomycin stimulation. This sustained activation of CAMK1D was completely abolished by Thr180Ala mutation and inhibited by CaM-KK inhibitor, STO-609, indicating a functional CaM-KK/ CAMK1D cascade in HeLa cells (2).
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| Storage: |
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—Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoid repeated thawing and freezing.
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| References: |
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—1. Verploegen,S., Ulfman,L., van Deutekom,H.W., van Aalst,C., Honing,H., Lammers,J.W., Koenderman,L. and Coffer,P.J. Characterization of the role of CaMKI-like kinase (CKLiK) in human granulocyte function. Blood 106 (3), 1076-1083 (2005) 2. Ishikawa,Y., Tokumitsu,H., Inuzuka,H., Murata-Hori,M., Hosoya,H. and Kobayashi,R. Identification and characterization of novel components of a Ca2+/calmodulin-dependent protein kinase cascade in HeLa cells. FEBS Lett. 550 (1-3), 57-63 (2003)
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