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Product Name
CAMK1b, active
Catalog Number
E-K008
Unit Price
$225.00
Quantity
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Recombinant full-length mouse CAMK1b containing N-terminal GST tag was expressed by baculovirus in Sf 9 insect cells.
The gene accession number is NM_012040.
This material is sold for research purposes only.
Specific Activity
1322 nmol phosphate incorporated into Autocamtide 2 per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
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| Scientific Background: |
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—Many of the effects of calcium are mediated via its interaction with calmodulin and the subsequent activation of Ca(2+)/calmodulin-dependent (CaM) kinases. CaM kinases are involved in a wide variety of cellular processes including muscle contraction, neurotransmitter release, cell cycle control, and transcriptional regulation. While CaMKII has been implicated in learning and memory, the biological role of the other multifunctional CaM kinases, CaMKI and CaMKIV, is largely unknown. CaMKIbeta, or pregnancy upregulated non-ubiquitously expressed CaM kinase (PNCK), is a 38-kDa serine/threonine kinase whose catalytic domain shares 45-70% identity with members of the CaM kinase family. The gene for CaMKIbeta localizes to mouse chromosome X. CaMKIbeta is upregulated during intermediate and late stages of murine fetal development with highest levels of expression in developing brain, bone, and gut. CaMKIbeta is also expressed in a tissue-specific manner in adult mice with highest levels of expression detected in brain, uterus, ovary, and testis. Interestingly, CaMKIbeta expression in these tissues is restricted to particular compartments and appears to be further restricted to subsets of cells within those compartments. The chromosomal localization of CaMKIbeta, along with its tissue-specific and restricted pattern of spatial expression during development, suggests that CaMKIbeta may be involved in a variety of developmental processes including development of the central nervous system (1). Also CaMKIbeta2, an isoform of mCaMKIbeta, was mainly identified in the nervous system, including brain, spinal cord, trigeminal ganglion, and retina. Within the CNS, the expression of CaMKIbeta2 is detected in the mantle zone, but not in the ventricular zone, suggesting its possible involvement in the differentiation of neurons (2).
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| Storage: |
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—Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoid repeated thawing and freezing.
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| References : |
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—1. Mitchell PJ, Barker KT, Martindale JE, Kamalati T, Lowe PN, Page MJ, Gusterson BA, Crompton MR.Cloning and characterisation of cDNAs encoding a novel non-receptor tyrosine kinase, brk, expressed in human breast tumours. Oncogene. 1994 Aug;9(8):2383-90. 2. Mitchell PJ, Barker KT, Shipley J, Crompton MR. Characterisation and chromosome mapping of the human non receptor tyrosine kinase gene, brk. Oncogene. 1997 Sep 18;15(12):1497-502.Erratum in: Oncogene 1998 Jul 9;17(1):129. 3. Barker KT, Jackson LE, Crompton MR. BRK tyrosine kinase expression in a high proportion of human breast carcinomas. Oncogene. 1997 Aug 14;15(7):799-805.
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