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Product Name
BLK, active
Catalog Number
E-K005
Unit Price
$225.00
Quantity
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Recombinant full-length human BLK containing N-terminal GST tag expressed by baculovirus in Sf 9 insect cells.
The gene accession number is BC007371.
This material is sold for research purposes only.
Specific Activity
579 nmol phosphate incorporated into Poly(Glu-Tyr) per minute per mg protein at 30°C using a final concentration of 50 µM ATP (0.83 µCi/assay).
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| Scientific Background: |
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—Blk, also known as B lymphoid kinase, is a 55 kd tyrosine kinases with a SH3, SH2 and catalytic domains that contain consensus sequences of the src protein tyrosine kinase family. Blk is expressed specifically in the B cell lineage and plays a role in signal transduction pathway that is restricted to B lymphoid cells (1). Stimulation of resting B-lymphocytes with antibodies to surface immunoglobulin (sIgD or sIgM) induces activation of Blk (2). Upon activation, the SH2 domain of Blk binds more than 10 distinct phosphoprotein including those of 72, 76, 115, and 130 kd (3). Blk prefers peptide substrates of the structure I/L-Y-D/E-X-L which resemble critical features of the ITAM motifs found in, e.g. the intracellular components Ig-alpha and Ig-beta of the beta cell receptor. Blk has a strong preference for a negatively charged amino acid in position +1 (4).
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| Storage: |
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—Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoid repeated thawing and freezing.
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| References : |
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—1. Dymecki SM, Niederhuber JE, Desiderio SV. Specific expression of a tyrosine kinase gene, blk, in B lymphoid cells. Science. 1990 Jan 19;247(4940):332-6.2. Burkhardt AL, Brunswick M, Bolen JB, Mond JJ. Anti-immunoglobulin stimulation of B lymphocytes activates src-related protein-tyrosine kinases. Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7410-4. 3. Malek SN, Desiderio S. SH2 domains of the protein-tyrosine kinases Blk, Lyn, and Fyn(T) bind distinct sets of phosphoproteins from B lymphocytes. J Biol Chem. 1993 Oct 25;268(30):22557-65. 4. Schmitz R, Baumann G, Gram H. Catalytic specificity of phosphotyrosine kinases Blk, Lyn, c-Src and Syk as assessed by phage display. J Mol Biol. 1996 Aug 2;260(5):664-77.
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