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Product Name
PIP3 Mass ELISA
Catalog Number
K-2500
Unit Price
$514.00
Quantity
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The production of PI(3,4,5)P3 from PI(4,5)P2 by type-1 PI 3-kinases (PI3-K) is important in multiple cell signaling pathways. Typically, experiments to measure PI 3-K activity have involved phosphorylation of a phosphoinositide substrate using 32P, then extraction of radioactive products, and separation using thin-layer chromatography. The assay plate method developed by Echelon Biosciences, Inc. allows the user to determine PI 3-K activity by measuring the amount of PI(3,4,5)P3 extracted from cells by means of a competitive ELISA format, eliminating the need for radioactive labeling of cells and thin layer chromatography.
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| Customer Testimonial: |
—"I have conducted several experiments measuring PIP3 levels in lymphocytes using Echelon's PIP3 mass kit. We found this assay system to meet our needs with respect to both sensitivity and reproducibility. Furthermore, the protocol is straightforward and can be completed more rapidly than other methods currently available to measure PIP3 levels in cells."
Bruce J. Shenker, Ph.D.
Associate Dean for Research, Chair, Department of Pathology
University of Pennsylvania School of Dental Medicine
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—PIP3 Mass Assays
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