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Product Name
PI 3-Kinase Fluorescence Polarization Kit, 384 assays
Catalog Number
K-1100
Unit Price
$529.00
Quantity
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The Phosphatidylinositol 3-Kinase (PI3-K) Fluorescence
Polarization assay determinations PI3-K activity through quantification of its
product, PI(3,4,5)P3.
Once PI 3-K reaction are complete, the PI(3,4,5)P3 detector protein and the
fluorescent probe are added. Polarization (mP) values decrease as probe, binding
to the PI(3,4,5)P3
detector, is displaced by the PI(3,4,5)P3
produced by enzymatic activity.
Class I Phosphoinositide 3-kinases
(PI3-K), are ubiquitously expressed lipid kinases which phosphorylate PI(4,5)P2
at the 3-hydroxyl of the inositol ring producing PtdIns(3,4,5)P3.
PtdIns(3,4,5)P3
has shown to be an important lipid second messenger with key roles in
fundamental cellular responses such as proliferation and survival. Due to these
interesting biological roles, PI3-K and PtdIns(3,4,5)P3
have become attractive targets for research and drug development in many
diseases including inflammation and cancer. Echelon has multiple formats
available for the determination of class I PI3-K activity. |
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| Volume Discount: |
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—Bulk Discounts available, please email echelon@echelon-inc.com for details
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| Benefits: |
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—Quick, stable and sensitive
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| Formats: |
—ELISA
—Fluorescence Polarization
—AlphaScreen™
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| Storage: |
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—2-8 °C, Do not freeze thaw multiple times
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| Applications: |
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—in vitro determination of PI3-K activity in cancer tissues and cell lines- Compound screening for drug discovery
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| Catalog: |
—PI 3-Kinase Assays
—PI3K
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